Structure of Fluorescein potassium salt CAS 6417-85-2

Iden­ti­fi­ca­tion

CAS Number

6417-85-2

Name

Flu­o­res­cein potas­si­um salt

Syn­onyms

dipotassium;3-oxospiro[2-benzofuran-1,9′-xanthene]-3′,6′-diolate

SMILES

C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)[O-])OC5=C3C=CC(=C5)[O-].[K+].[K+]

Std­InChI

InChI=1S/C20H12O5.2K/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

Std­InChIKey

DZSPM­L­RD­MGJ­­VAT-UHF­F­­FAOYSA-L

Mol­e­c­u­lar Formula

C20H13KO5

Mol­e­c­u­lar Weight

408.5

EINECS

229-143-9

Prop­er­ties

Appear­ance

Pale yel­low solid

Sol­u­bil­i­ty

Sol­u­ble in water, DMSO, DMF, poor­ly sol­u­ble in dichloromethane, petro­le­um ether

Sol­u­bil­i­ty (sol­vent : water, tem­per­a­ture : 25℃)

35 mg/​mL

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Flu­o­res­cein potas­si­um salt CAS 6417-85-2

Iden­ti­fi­ca­tion Methods

HNMR, HPLC

Puri­ty

96% min

Max­i­mum absorp­tion wavelength

328 nm

Max­i­mum emis­sion wavelength

531 nm

Shelf Life

2 years

Stor­age

Under room tem­per­a­ture away from light

Fea­tures

  1. The flu­o­res­cence is strongest under alka­line con­di­tions, show­ing green fluorescence ;
  2. Usu­al­ly used in aque­ous solu­tion (such as 40% aque­ous solution);
  3. Rel­a­tive­ly high safe­ty, envi­ron­men­tal­ly friend­ly when used in small amounts.

Known Appli­ca­tion

  1. Dyes and trac­ers
    Flu­o­res­cent trac­er :
    Com­mon­ly used to detect liq­uid flow path, leak­age loca­tion, ground­wa­ter flow direc­tion, water pipe leak­age, etc.
    Water pol­lu­tion track­ing :
    In the field of envi­ron­men­tal pro­tec­tion, it is used to track the dif­fu­sion path of pol­lu­tants in water bodies.
  2. Bio­med­ical and ana­lyt­i­cal appli­ca­tions
    Cell stain­ing agent :
    Used as a flu­o­res­cent dye when observ­ing cells, tis­sues or bio­log­i­cal struc­tures under a micro­scope.
    Oph­thal­mo­log­i­cal exam­i­na­tion :
    Used for corneal stain­ing test to check corneal dam­age or for­eign mat­ter (such as used with a blue light slit lamp).
    Cap­il­lary elec­trophore­sis, elec­trophore­sis analy­sis :
    As a flu­o­res­cent label­ing reagent to improve detec­tion sensitivity.
  3. Indus­tri­al detec­tion
    Pipeline detec­tion :
    Adding this dye to petro­chem­i­cal, refrig­er­a­tion, air con­di­tion­ing and oth­er sys­tems, and irra­di­at­ing with ultra­vi­o­let light can locate the leak point.
    CIP val­i­da­tion :
    In the phar­ma­ceu­ti­cal and food indus­tries, it is used to con­firm whether the clean­ing process is thorough.

Gen­er­al View of Documents

HNMR of Fluorescein potassium salt CAS 6417-85-2
HNMR of Flu­o­res­cein potas­si­um salt CAS 6417-85-2
HPLC of Fluorescein potassium salt CAS 6417-85-2
HPLC of Flu­o­res­cein potas­si­um salt CAS 6417-85-2
ESI-MS of Fluorescein potassium salt CAS 6417-85-2
ESI-MS of Flu­o­res­cein potas­si­um salt CAS 6417-85-2
Absorption Spectrum of Fluorescein potassium salt CAS 6417-85-2
Absorp­tion Spec­trum of Flu­o­res­cein potas­si­um salt CAS 6417-85-2
Emission spectrum of Fluorescein potassium salt CAS 6417-85-2
Emis­sion spec­trum of Flu­o­res­cein potas­si­um salt CAS 6417-85-2

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd (https://​www​.wat​son​-bio​.com/).

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