
Identification
CAS Number
6417-85-2
Name
Fluorescein potassium salt
Synonyms
dipotassium;3-oxospiro[2-benzofuran-1,9′-xanthene]-3′,6′-diolate
SMILES
C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)[O-])OC5=C3C=CC(=C5)[O-].[K+].[K+]
StdInChI
InChI=1S/C20H12O5.2K/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
StdInChIKey
DZSPMLRDMGJVAT-UHFFFAOYSA-L
Molecular Formula
C20H13KO5
Molecular Weight
408.5
EINECS
229-143-9
Properties
Appearance
Pale yellow solid
Solubility
Soluble in water, DMSO, DMF, poorly soluble in dichloromethane, petroleum ether
Solubility (solvent : water, temperature : 25℃)
35 mg/mL
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our Fluorescein potassium salt CAS 6417-85-2
Identification Methods
HNMR, HPLC
Purity
96% min
Maximum absorption wavelength
328 nm
Maximum emission wavelength
531 nm
Shelf Life
2 years
Storage
Under room temperature away from light
Features
- The fluorescence is strongest under alkaline conditions, showing green fluorescence ;
- Usually used in aqueous solution (such as 40% aqueous solution);
- Relatively high safety, environmentally friendly when used in small amounts.
Known Application
- Dyes and tracers
Fluorescent tracer :
Commonly used to detect liquid flow path, leakage location, groundwater flow direction, water pipe leakage, etc.
Water pollution tracking :
In the field of environmental protection, it is used to track the diffusion path of pollutants in water bodies. - Biomedical and analytical applications
Cell staining agent :
Used as a fluorescent dye when observing cells, tissues or biological structures under a microscope.
Ophthalmological examination :
Used for corneal staining test to check corneal damage or foreign matter (such as used with a blue light slit lamp).
Capillary electrophoresis, electrophoresis analysis :
As a fluorescent labeling reagent to improve detection sensitivity. - Industrial detection
Pipeline detection :
Adding this dye to petrochemical, refrigeration, air conditioning and other systems, and irradiating with ultraviolet light can locate the leak point.
CIP validation :
In the pharmaceutical and food industries, it is used to confirm whether the cleaning process is thorough.
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This product is developed by our R&D company Watson Bio Ltd (https://www.watson-bio.com/).
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