Overview

Gen­er­al Description

Recom­bi­nant Human B7-H5/​Gi24/​VISTA Pro­tein is expressed from HEK293 with hFc tag at the C​-ter​mi​nal​.It con­tains Phe33-Ala194.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

B7-H5/​Gi24/​VISTA

Syn­onyms

B7H5 ; B7-H5 ; VISTA ; VSIR ; PD1H ; PD-1H ; 4632428N05Rik ; C10orf54 ; Dies1 ; Gi24 ; PP2135 ; SISP1

Acces­sion

Q9H7M9

Species

Human

Exact Sequence

Phe33-Ala194

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 44.9 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human B7-H5, hFc Tag at 0.5ug/ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Anti-B7-H5 Anti­body, hFc Tag with the EC50 of 8.0ng/ml deter­mined by ELISA (QC Test). The affin­i­ty con­stant of 14.44 uM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

C-hFc

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 5% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Human B7-H5, hFc Tag cap­tured on CM5 Chip via Pro­tein A can bind Human IGSF11, His Tag with an affin­i­ty con­stant of 14.44 uM as deter­mined in SPR assay (Bia­core T200).

Immo­bi­lized Human B7-H5, hFc Tag at 0.5ug/ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Anti-B7-H5 Anti­body, hFc Tag with the EC50 of 8.0ng/ml deter­mined by ELISA (QC Test).

The puri­ty of Human B7-H5 is greater than 95% as deter­mined by SEC-HPLC

Human B7-H5 on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

Quick Inquiry

Fill out our inquiry form and one of our experts will be in touch with you shortly.

























    Please prove you are human by select­ing the truck.