Overview

Gen­er­al Description

Recom­bi­nant Human IL-7 Pro­tein is expressed from E.coli with­out tag​.It con­tains Asp26-His177.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

IL-7

Syn­onyms

IL7 ; Inter­leukin-7 ; IL-7 ; Lym­phopoi­etin-1 ; PBGF

Acces­sion

P13232

Species

Human

Exact Sequence

Asp26-His177

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 17.5 kDa same as Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human IL-7 at 0.5ug/ml (100ul/​Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml deter­mined by ELISA. The affin­i­ty con­stant of 22.66 nM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

No Tag

Express Sys­tem

E.coli

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in 25mM PB, 250mM NaCl (pH 6.8). Nor­mal­ly 8% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in 25mM PB, 250mM NaCl (pH 6.8).

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Human IL-7R alpha, His Tag cap­tured on CM5 Chip via anti-his anti­body can bind Human IL-7, No Tag with an affin­i­ty con­stant of 22.66 nM as deter­mined in SPR assay (Bia­core T200).

Immo­bi­lized Human IL-7 at 0.5ug/ml (100ul/​Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml deter­mined by ELISA.

Human IL-7, No Tag stim­u­lates pro­lif­er­a­tion of PHA-pre­treat­ed human periph­er­al blood mononu­clear cell (PBMC) with the EC50 for this effect is 3.5ng/mL. The spe­cif­ic activ­i­ty of Recom­bi­nant Human IL-7 is >1.0 x 108 IU/​mg, which is cal­i­brat­ed against human IL-7 WHO Inter­na­tion­al Stan­dard (NIB­SC code : 90/530) (QC tested).

The puri­ty of Human IL-7 is greater than 95% as deter­mined by SEC-HPLC.

Human IL-7 on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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