20210526150116 600x400 - Human NKG2A/CD159a Protein, Accession: P26715

Overview

Gen­er­al Description

Recom­bi­nant Human NKG2A/​CD159a Pro­tein is expressed from HEK293 with His tag and Avi tag at the N​-ter​mi​nal​.It con­tains Arg100-Leu233.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

NKG2A/​CD159a

Syn­onyms

NKG2A ; NKG2-A ; CD94 ; KLRD1 ; KP43 ; KLRC1 ; CD159A

Acces­sion

P26715

Species

Human

Exact Sequence

Arg100-Leu233

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 18.3 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human NKG2A, His Tag at 5ug/​ml (100ul/​Well) on the plate. Dose response curve for Anti-NKG2A Anti­body, hFc Tag with the EC50 of 0.51ug/ml deter­mined by ELISA (QC Test). The affin­i­ty con­stant of 0.23uM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

N-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 90% as deter­mined by Tris-Bis PAGE

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 5% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

20210526150116 - Human NKG2A/CD159a Protein, Accession: P26715

Anti-NKG2A anti­body cap­tured on Pro­tein A chip, can bind Human NKG2A, His Tag with an affin­i­ty con­stant of 0.23uM as deter­mined in a SPR assay (Bia­core T200).

20230407153907 - Human NKG2A/CD159a Protein, Accession: P26715

Immo­bi­lized Human NKG2A, His Tag at 5ug/​ml (100ul/​Well) on the plate. Dose response curve for Anti-NKG2A Anti­body, hFc Tag with the EC50 of 0.51ug/ml deter­mined by ELISA (QC Test).

1605259124356648(1) - Human NKG2A/CD159a Protein, Accession: P26715

Human NKG2A on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 90%.

validation images - Human NKG2A/CD159a Protein, Accession: P26715

validation images - Human NKG2A/CD159a Protein, Accession: P26715

validation images - Human NKG2A/CD159a Protein, Accession: P26715

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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