Overview

General Description

Recombinant Human NKp46/NCR1/CD335 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminal.It contains Gln22-Asn255.

Biotinylated

Specifications

Target

NKp46/NCR1/CD335

Synonyms

CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094

Accession

O76036

Species

Human

Exact Sequence

Gln22-Asn255

Molecular Weight

The protein has a predicted MW of 52.9 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human NKp46, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nkp46 Antibody, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-mFc

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

General View of Documents

Immobilized Human NKp46, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nkp46 Antibody, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA.

The purity of Human NKp46 is greater than 95% as determined by SEC-HPLC.

Human NKp46 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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