Structure of Monoamine Oxidase CAS 9001 66 5 - KAOD(Monoamine Oxidase) CAS 9001-66-5

Iden­ti­fi­ca­tion

CAS Number

9001-66-5

Name

Monoamine Oxi­dase

EC Number

1.5.3 (Recom­bi­nant from microorganism)

Mol­e­c­u­lar Formula

C7H15N2O6P 

Mol­e­c­u­lar Weight

55 kDa (SDS-PAGE)

EINECS

232-623-0

Safe­ty Data

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Monoamine Oxi­dase CAS 9001-66-5

Spec­i­fi­ca­tion Stan­dards
Appear­anceYel­low amor­phous pow­der, lyophilized
Pro­tein purity≥90% (from SDS-PAGE)
Activ­i­ty≥4 U/​mg solid
Cata­lase≤0.001%
ATPase≤0.005%
Glu­cose oxidase≤0.03%
Cho­les­terol oxidase≤0.003%

Gen­er­al Assay Characteristics 

Method
Enzymatic/​RATE A/E
ReagentsReady to use
Cal­i­bra­torsLev­el 1-0.0 U/L ; Lev­el 1-50 U/L
Cal­i­bra­tion Stability28 days
Sam­ple TypeEDTA,SST,Serum,Heparinised plas­ma
Assay Range0-200U/L undi­lut­ed
Stan­dard­i­s­a­tion

Assay Prin­ci­ple

1 - KAOD(Monoamine Oxidase) CAS 9001-66-5

The assay is based on the increase in absorbance at 555 nm as the for­ma­tion of quinoneimine dye pro­ceeds in the for­ward reactions.

Unit Def­i­n­i­tion

One unit (U) is defined as the amount of enzyme which pro­duces 1 μmol of H2O2 per min under the con­di­tions described below.

Pro­posed MAO Recipe 

Reagent I

Com­po­si­tionCon­cen­tra­tionCat­a­log Number
Tris-HCl buffer50mM
NADH0.45mM CS001
α-Ketog­lu­­tarate15mM
Deter­gent, Preservative

Reagent II

Com­po­si­tionCon­cen­tra­tionCat­a­log Number
Tris-HCl buffer50mM
Ben­zy­lamine 10mM
Detergent,Preservative

Major reagent ingre­di­ents made by Assay Spe­cilist Lab are list on right

Stan­dard&Con­trol

Home Made : MAO in buffer

Reac­tion curve chart 

Calibrator 1 1 - KAOD(Monoamine Oxidase) CAS 9001-66-5
Calibrator 2 1 - KAOD(Monoamine Oxidase) CAS 9001-66-5

Known Appli­ca­tion

The enzyme is use­ful for the deter­mi­na­tion of fruc­­to­­syl-L-amino acid.

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd(http://​www​.ulcho​.com/), and here is the cor­re­spond­ing linkhttp://​www​.ulcho​.com/​m​o​n​o​a​m​i​n​e​-​o​x​i​d​a​s​e​-​c​a​s​-​9​0​0​1​-​6​6​-5/

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