mRNA Cap-2′-O-Methyltransferase CAS 61970-02-3

• Iden­ti­fi­ca­tion
• Prop­er­ties
• Safe­ty Data
• Spec­i­fi­ca­tions & Oth­er Information
• Links
• Quick Inquiry

Iden­ti­fi­ca­tion

CAS Number

61970-02-3

EC Number

2.1.1.57

Name

mRNA Cap-2′-O-Methyltransferase

Prop­er­ties

Opti­mal reac­tion temperature

37℃

Def­i­n­i­tion of active unit

Def­i­n­i­tion of active unit : 1 active unit is defined as the amount of enzyme need­ed to incor­po­rate 10 pmol of (α32P)GTP into 80nt tran­script with­in 1h at 37℃ .

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our mRNA Cap-2′-O-Methyltransferase CAS 61970-02-3

Prod­uct Introduction

This prod­uct is the mRNA Cap-2′-O-Methyltransferase of vac­cinia virus recom­bi­nant­ly expressed in E. coli. With the RNA of 7-methyl­guano­­sine cap (Cap0) as the sub­strate, it specif­i­cal­ly trans­fers the methyl of methy­la­tion dona­tor SAM to the Cap0 struc­ture in RNA to form the Cap1 struc­ture, which can enhance the trans­la­tion effi­cien­cy of mRNA and avoid the innate immune response in cer­tain cells.

Prod­uct Features

In vit­ro cap­ping can avoid in vivo immune response.

Prod­uct Composition

10× Cap­ping Buffer con­tains : 0.5M Tris-HCl(pH8.0), 50mM KCl, 10mM MgCl2 and 10mM DTT.

Qual­i­ty Control

Puri­ty ≥ 95%, resid­ual host cell DNA ≤ 100pg/​mg, resid­ual host cell pro­tein ≤ 50ppm, resid­ual endo­tox­in ≤10EU/​mg, no resid­ual RNase, endonu­cle­ase, exonu­cle­ase or pro­tease, germ-free, pathogen-free.

Links

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd (http://​www​.ulcho​.com/).

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