Poly(A) Poly­merase CAS 9026-30-6

• Iden­ti­fi­ca­tion
• Prop­er­ties
• Safe­ty Data
• Spec­i­fi­ca­tions & Oth­er Information
• Links
• Quick Inquiry

Iden­ti­fi­ca­tion

CAS Number

9026-30-6

EC Number

2.7.7.19

Name

Poly(A) Poly­merase

Syn­onyms

Polynu­cleotide Adeny­lyl­trans­ferase
ATP:polynu­cleotide adeny­lyl­trans­ferase
ATP RNA Adeny­lyl­trans­ferase
ATP-RNA Adeny­lyl­trans­ferase
Adeny­lyl­trans­ferase, ATP-RNA
Adeny­lyl­trans­ferase, polynu­cleotide
Poly A Poly­merase
Polyadeny­late poly­merase
Polyadeny­late synthetase

MDL Number

MFCD00166322

Prop­er­ties

Opti­mal reac­tion temperature

37℃

Def­i­n­i­tion of active unit

Def­i­n­i­tion of active unit : 1 active unit is defined as the amount of enzyme need­ed to incor­po­rate 1 nmol of AMP into RNA with­in 10min at 37℃ in a 20μL reac­tion system.

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Poly(A) Poly­merase CAS 9026-30-6

Prod­uct Introduction

This prod­uct is the Poly(A) Poly­merase recom­bi­nant­ly expressed in E. coli. It cat­alyzes with ATP as the sub­strate, and adds adenyl acid to the 3’-hydroxyl ter­mi­nus of RNA to form the PolyA tail struc­ture in the form of AMP.

Prod­uct Features

Effi­cient mRNA cap­ping : adding 200nt polyA tail to 10μg of RNA in 10min (5U enzyme for cap­ping). Homoge­nous tail­ing length : con­trol­ling polyA tail length by adjust­ing reac­tion time or enzyme dosage.

Prod­uct Composition

1x Reac­tion Buffer con­tains 0.5M Tris-HCl(pH8.0), 50mM KCl and 10mM MgCl2.

Qual­i­ty Control

Puri­ty ≥ 95%, resid­ual host cell DNA ≤ 100pg/​mg, resid­ual host cell pro­tein ≤ 50ppm, resid­ual endo­tox­in ≤10EU/​mg, no resid­ual RNase, endonu­cle­ase, exonu­cle­ase or pro­tease, germ-free, pathogen-free.

Links

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd (http://​www​.ulcho​.com/).

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