Structure of Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025 24 5 - Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025-24-5

Iden­ti­fi­ca­tion

CAS Number

9025-24-5

EC Number

3.4.17.2

Name

Recom­bi­nant Car­boxypep­ti­dase B

Syn­onyms

N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phenyl-2-propanyl]prolinamid [Ger­man] [ACD/IUPAC Name]
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phenyl-2-propanyl]prolinamide [ACD/IUPAC Name]
N-(3-Carboxypropanoyl)alanylalanyl-N-[1-(benzylsulfanyl)-1-oxo-3-phényl-2-propanyl]prolinamide [French] [ACD/IUPAC Name]
Pro­li­namide, N-(3-carboxy-1-oxopropyl)alanylalanyl-N-[2-oxo-1-(phenylmethyl)-2-[(phenylmethyl)thio]ethyl]- [ACD/​Index Name]
3-[(1-{[1-(2-{[1-(BEN­ZYL­SUL­FANYL)-1-OXO-3-PHENYL­PROPAN-2-YL]CAR­BAMOYL}PYRRO­LIDIN-1-YL)-1-OXO­PROPAN-2-YL]CAR­BAMOYL}ETH­YL)CAR­BAMOYL]PROPANOIC ACID
4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid
9025-24-5 [RN]
Car­boxypep­ti­dase B from human pan­creas
Car­boxypep­ti­­dase- B porcine pan­creas
Pro­t­a­m­i­nase

SMILES

CC(C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(Cc2ccccc2)C(=O)SCc3ccccc3)NC(=O)CCC(=O)O

InChi

InChI=1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)

InChIKey

TWURVFFN­OD­FJBJ-UHF­F­­FAOYSA-N

Mol­e­c­u­lar Formula

C31H38N4O7S

Mol­e­c­u­lar Weight

610.721

EINECS

232-788-9

MDL Number

MFCD00081476

Prop­er­ties

Appear­ance

White or off white, or yel­low­ish powder

Safe­ty Data

Sym­bol

exclamation mark jpg - Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025-24-5health hazard jpg - Recombinant Carboxypeptidase B EC 3.4.17.2 CAS 9025-24-5GHS07,GHS08

Sig­nal Word

Dan­ger

Haz­ard statements

H315H319H334H335

Pre­cau­tion­ary Statements

P302 + P352P305 + P351P338

Tar­get organs

Res­pi­ra­to­ry system

Per­son­al Pro­tec­tive Equipment

Eye­shields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Recom­bi­nant Car­boxypep­ti­dase B EC 3.4.17.2 CAS 9025-24-5

ItemsRecom­bi­nant Car­boxypep­ti­dase BSequenc­ing Grade Car­boxypep­ti­dase B
Spe­cif­ic activity≥170 units/​mg pro.≥200 units/​mg pro.
SDS-PAGESin­gle major bandSin­gle major band
Mol­e­c­u­lar Weight(SDS-PAGE)33.8±3.4 kDa33.8±3.4 kDa
Puri­ty(HPLC)/≥ 95%
Unit def­i­n­i­tionOne unit of car­boxypep­ti­dase B activ­i­ty hydrolyzes one micro­mole ofhip­puryl-L-argi­­nine per minute at 25℃, pH 7.65.Pre­pare 1-10mg/ml car­boxypep­ti­dase B with ster­ile water or 25mM Tris-HCl pH 7.65. The ratio to aimed pro­tein is 1:50 to 1:1000 (w/​w), the opti­mum pH is pH 7.5-9.0.
Rec­om­mend­ed usagePre­pare 1-10mg/ml recom­bi­nant car­boxypep­ti­dase B with ster­ile water or 25mM Tris-HCl pH 7.65. The ratio to aimed pro­tein is 1:50 to 1:1000 (w/​w), the opti­mum pH is pH 7.5-9.0./

Descrip­tion

Car­boxypep­ti­dase B cat­alyzes hydrol­y­sis of the basic amino acids lysine, argi­nine and his­ti­dine from the C-ter­mi­­nal end of polypep­tides. The mol­e­c­u­lar weight is 33.8kD, pI is 6.0 and the opti­mum pH is 7.5-9.0. Car­boxypep­ti­dase B is com­pet­i­tive­ly inhib­it­ed by argi­nine and lysine. The enzyme is also inhib­it­ed by met­al chelat­ing agents, e.g., EDTA.

Source

It is a genet­i­cal­ly engi­neered pro­tein expressed in E.coli

Pro­tein Sequence

The amino acid sequence of recom­bi­nant car­boxypep­ti­dase B is iden­ti­cal to rat car­boxypep­ti­dase B

Sta­bil­i­ty of storage

Sequenc­ing grade car­boxypep­ti­dase B lyophilized should be stored under 2-8℃ in sealed con­tain­er. It is sta­ble with­in 24 months.After dis­solved with ster­ile water or 25mM Tris-HCl, it should be stored under -20℃. Avoid repeat­ed freez­ing and thawing.

Sta­bil­i­ty of transport

The prod­uct is sta­ble by blue ice insu­la­tion transport.

Advan­tage

  1. Ani­mal ori­gin free : recom­bi­nant trypsin is no exoge­nous virus contamination,and any ani­mal ori­gin mate­r­i­al is not used in the pro­duc­tion process.
  2. Sta­ble qual­i­ty : Mass pro­duc­tion can ensure sta­ble and con­tin­u­ous batch pro​duc​tion​.It is no dif­fer­ence between the batch and the prod­uct qual­i­ty is stable.
  3. High puri­ty : High­er spe­cif­ic activ​i​ty​.Host pro­tein residues is less than the lim­its of bio­log­i­cal products.
  4. Lyophilized pow­der : The prod­uct is lyophilized pow­der and is easy to store and transport.

Known Appli­ca­tion

The pro­duc­tion of recom­bi­nant insulin and its ana­logues ; the qual­i­ty test of recom­bi­nant anti­bod­ies ; the deter­mi­na­tion of C-ter­mi­­nal amino acids of pro­teins ; the pro­duc­tion of oth­er recom­bi­nant polypep­tides ; and the enzy­mat­ic syn­the­sis of cer­tain spe­cif­ic com­pounds. Deter­mi­na­tion of pro­tein C-ter­mi­­nal basic amino acids such as argi­nine, lysine and his­ti­dine ; detec­tion of anti­body basic peak.

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd(https://​www​.wat​son​-bio​.com/), and here is the cor­re­spond­ing linkhttps://​www​.wat​son​-bio​.com/​r​e​c​o​m​b​i​n​a​n​t​-​s​o​y​b​e​a​n​-​t​r​y​p​s​i​n​-​i​n​h​i​b​i​t​o​r​-​c​a​s​-​9​0​3​5​-​8​1​-8/

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