sds page 109 04 3 - Recombinant Human Beta-defensin 4 (rHuBD-4) CAS 109-04-1816

Offi­cial Full Name

Recom­bi­nant Human Beta-defensin 4 (rHuBD-4)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

109-04-1816

Syn­onyms

Defensin beta4

Squence

region 109 04 1 - Recombinant Human Beta-defensin 4 (rHuBD-4) CAS 109-04-1816

Amino Acid Sequence

EFELDRIC­GY GTAR­CRKKCR SQEYRI­GR­CP NTY­AC­CLRKW DESLL­NRTKP

Syn­onyms

Defensin beta4

Acces­sion

Q8WTQ1

Gene ID

140596

Sum­ma­ry

Defensins (alpha and beta) are cation­ic pep­tides with antimi­cro­bial activ­i­ty against Gram-neg­a­­tive and Gram-pos­i­­tive bac­te­ria, fun­gi, and enveloped virus­es. They are 2-6 kDa pro­teins and take impor­tant roles in innate immune sys­tem. On the basis of their size and pat­tern of disul­fide bond­ing, mam­malian defensins are clas­si­fied into alpha, beta and theta cat­e­gories. β-defensins con­tain a six-cys­teine motif that forms three intra-mol­e­c­u­lar disul­fide bonds. Four human β-defensins have been iden­ti­fied and they are expressed on some leuko­cytes and at epithe­lial sur­faces. Because β-defensins is cation­ic pep­tides, they can there­fore inter­act with the mem­brane of invad­ing microbes, which are neg­a­tive due to lipopolysac­cha­rides (LPS) and lipote­i­choic acid (LTA) found in the cell mem­brane. Espe­cial­ly, they have high­er affin­i­ty to the bind­ing site com­pared to Ca2+ and Mg2+ ions. Fur­ther­more, they can affect the sta­bil­i­ty of the mem­brane.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 6.0 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 50 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The bio­log­i­cal activ­i­ty deter­mined by a chemo­taxis bioas­say using human mono­cytes is in a con­cen­tra­tion range of 0.1-100.0 ng/​ml.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in 20 mM PB, pH 7.4, 130 mM NaCl.

Endo­tox­in

Less than 1 EU/​ug of rHuBD-4 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 98 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

sds page 109 04 2 - Recombinant Human Beta-defensin 4 (rHuBD-4) CAS 109-04-1816

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Beta-defensin 4 (rHuBD-4) on ChemWhat

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