Official Full Name
Recombinant Human Beta-defensin 4 (rHuBD-4)
CAS Number (Or Watson Number for Non-CAS Products)
Amino Acid Sequence EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP
Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS
) and lipoteichoic acid (LTA
) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.
Approximately 6.0 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB
, pH 7.4, 130 mM NaCl.
Less than 1 EU
/ug of rHuBD-4 as determined by LAL
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA
to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
> 98 % by SDS-PAGE
Safety Data Sheet (SDS) Download
Click to download
Technical Data Sheet (TDS) Download
Click to download
This product is branded by ChemWhat and sold through our website and here is the corresponding link Recombinant Human Beta-defensin 4 (rHuBD-4) on ChemWhat
Fill out our inquiry form and one of our experts will be in touch with you shortly.