Recom­bi­nant Human Fibrob­last Growth Fac­tor 9 (rHuFGF-9) CAS 104-09-1816

Offi­cial Full Name

Recom­bi­nant Human Fibrob­last Growth Fac­tor 9 (rHuFGF-9)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

104-09-1816

Syn­onyms

GAF, Glia-acti­­vat­ing fac­tor, HBGF-9

Squence

Amino Acid Sequence

APLGEVGNYF GVQ­DAVPFGN VPVLPVD­SPV LLS­DHLGQSE AGGL­PRG­PAV TDLDHLKGIL RRRQ­LY­CRTG FHLEIF­P­NGT IQGTRKDHSR FGILE­FISIA VGLVSIRGVD SGLYL­GM­NEK GELYGSEKLT QECVFRE­QFE ENWYN­TYSSN LYKHVDT­GRR YYVALNKDGT PREGTRTKRH QKFTH­FL­PRP VDPD­KVPE­LY KDIL­SQS

Syn­onyms

GAF, Glia-acti­­vat­ing fac­tor, HBGF-9

Acces­sion

P31371

Gene ID

2254

Sum­ma­ry

Fibrob­last growth fac­­tor-9 (FGF-9) is a mem­ber of the fibrob­last growth fac­tor (FGF) fam­i­ly. All FGF fam­i­ly mem­bers are heparin bind­ing growth fac­tors with a core 120 amino acid (a.a.) FGF domain that allows for a com­mon ter­tiary struc­ture. FGF-9 plays an impor­tant role in the reg­u­la­tion of embry­on­ic devel­op­ment, cell pro­lif­er­a­tion, cell dif­fer­en­ti­a­tion and cell migra­tion. This pro­tein was iso­lat­ed as a secret­ed fac­tor that exhibits a growth-stim­u­lat­ing effect on cul­tured glial cells. FGF-9 is a monomer and inter­acts with FGFR1, FGFR2, FGFR3 and FGFR4. The human FGF-9 shares 98 % a.a. sequence iden­ti­ty with mouse, rat, equine, porcine, and bovine FGF-9.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 23.3 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 207 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by thymi­dine uptake assay using FGF-recep­­tors trans­fect­ed BaF3 cells is less than 0.5 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 2.0 × 106 IU/​mg.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in PBS, pH 7.4.

Endo­tox­in

Less than 1 EU/​ug of rHuFGF-9 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in 1 × PBS to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 95 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Fibrob­last Growth Fac­tor 9 (rHuFGF-9) on ChemWhat

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