Recom­bi­nant Human Liv­er-Expressed Chemokine/​CCL16 (rHuLEC/​CCL16) CAS 204-16-1816

Offi­cial Full Name

Recom­bi­nant Human Liv­er-Expressed Chemokine/​CCL16 (rHuLEC/​CCL16)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

204-16-1816

Syn­onyms

HCC-4, LCC-1, LMC, MTN-1, NCC-4

Squence

Amino Acid Sequence

QPKVPEWVNT PSTC­CLKYYE KVL­PRRLVVG YRKALNCHLP AIIFVTKRNR EVCT­NPND­DW VQEYIKDPNL PLLP­TRNLST VKI­ITAKNGQ PQLLNSQ

Syn­onyms

HCC-4, LCC-1, LMC, MTN-1, NCC-4

Acces­sion

O15467

Gene ID

6360

Sum­ma­ry

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 11.2 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 97 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The bio­log­i­cal activ­i­ty deter­mined by a chemo­taxis bioas­say using human mono­cytes is in a con­cen­tra­tion range of 10-100 ng/​ml.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in 20 mM PB, pH 7.4, 150 mM NaCl.

Endo­tox­in

Less than 1 EU/​ug of rHuLEC/​CCL16 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

1. Hedrick JA, Helms A, Vic­ari A, et al. 1998. Blood, 91 : 4242-7.2. Nomiya­ma H, Fuku­da S, Iio M, et al. 1999. J Inter­fer­on Cytokine Res, 19 : 227-34.3. Youn BS, Zhang S, Brox­mey­er HE, et al. 1998. Biochem Bio­phys Res Com­mun, 247 : 217-22.4. Kim IS, Jang SW, Sung HJ, et al. 2005. FEBS Lett, 579 : 6044-8.5. Fran­ci­ca G, Petro­lati A, Di Sta­sio E, et al. 2012. Acta Radi­ol, 53 : 394-400.

Puri­ty

> 97 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Liv­er-Expressed Chemokine/​CCL16 (rHuLEC/​CCL16) on ChemWhat

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