Ubiquitin-conjugating enzyme E2 K belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2K gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2K has been shown to interact with Huntingtin and RNF2. Additionally, it has been implicated in the degradation of huntingtin and suppression of apoptosis.
Source
Escherichia coli.
Molecular Weight
Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids (a.a.) of human UBE2K and 8 a.a. vector sequence including 6 × His tag at N-terminus.
Biological Activity
No data available
Appearance
Sterile Colorless liquid.
Formulation
A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 8.0, with 100 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.
Endotoxin
Less than 1 EU/ug of rHuUBE2K, His as determined by LAL method.
Reconstitution
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.
