Recom­bi­nant PreScis­sion Pro­tease (rPPase) CAS 461-03-1816

Offi­cial Full Name

Recom­bi­nant PreScis­sion Pro­tease (rPPase)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

461-03-1816

Syn­onyms

3C pro­tease, Picor­nain 3C, PSP

Squence

Amino Acid Sequence

Syn­onyms

3C pro­tease, Picor­nain 3C, PSP

Acces­sion

Gene ID

Sum­ma­ry

PreScis­sion pro­tease is a cys­teine pro­tease derived from human rhi­novirus – HRV3C Pro­tease. rPP is a fusion pro­tein of glu­tathione S-tran­s­ferase (GST) and human rhi­novirus (HRV) type 14 3C pro­tease. It specif­i­cal­ly rec­og­nizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues. Sub­strate recog­ni­tion and cleav­age are like­ly to be depen­dent not only upon pri­ma­ry struc­tur­al sig­nals, but also upon the super struc­tures of the fusion pro­tein. rPP works most effec­tive at 4°C and can digest sub­strates at room tem­per­a­ture as well.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

PreScis­sion Pro­tease is a fusion pro­tein of glu­tathione S-tran­s­ferase (GST) and human rhi­novirus (HRV) type 14 3C pro­tease. The pro­tease specif­i­cal­ly rec­og­nizes a sub­set of sequences which include the core amino acid sequence Leu-Phe-Gln/G­­ly-Pro cleav­ing between the Gln and Gly residues. Sub­strate recog­ni­tion and cleav­age are like­ly to be depen­dent not only upon pri­ma­ry struc­tur­al sig­nals, but also upon the sec­ondary and ter­tiary struc­tures of the fusion pro­tein as well.

Bio­log­i­cal Activity

Appear­ance

Ster­ile col­or­less liq­uid.

For­mu­la­tion

50 mM Tris-HCl, pH 7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithio­thre­itol. Chill to 5 °C pri­or to use.

Endo­tox­in

Recon­sti­tu­tion

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as sup­plied.- 3 months, -20 to -70 °C under ster­ile con­di­tions after open­ing.

Ref­er­ences

1. Wern­er G, Rosen­wirth B, Bauer E, et al. 1986. J Virol, 57 : 1084-93.2. Lib­by RT, Cos­man D, Cooney MK, et al. 1988. Bio­chem­istry, 27 : 6262-8.3. Aschauer B, Wern­er G, McCray J, et al. 1991. Virol­o­gy, 184 : 587-94.4. Leong LE, Walk­er PA, Porter AG. 1993. J Biol Chem, 268 : 25735-9.

Puri­ty

One unit is defined as the amount of enzyme need­ed to cleave 100 ug of fusion pro­tein in 16 hours to 90 % com­ple­tion at 5 °C in a buffer con­tain­ing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

SDS-PAGE

Pack Size

100IU/250IU

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

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Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant PreScis­sion Pro­tease (rPPase) on ChemWhat

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