sds page 145 08 3 361x400 - Recombinant Rat Heparin-binding EGF-like Growth Factor (rRtHB-EGF) CAS 145-08-1816

Offi­cial Full Name

Recom­bi­nant Rat Heparin-bind­ing EGF-like Growth Fac­tor (rRtHB-EGF)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

145-08-1816

Syn­onyms

HBEGF, DT-R

Squence

region 145 08 1 - Recombinant Rat Heparin-binding EGF-like Growth Factor (rRtHB-EGF) CAS 145-08-1816

Amino Acid Sequence

DLEGT­DLDLF KVAF­SSKPQA LATPGKEKNG KKKRKGKGLG KKRD­P­CLKKY KDY­CI­HGE­CR YLKEL­RIP­SC HCLP­GY­HGQR CHGLTL

Syn­onyms

HBEGF, DT-R

Acces­sion

Q06175

Gene ID

25433

Sum­ma­ry

Heparin-bind­ing epi­der­mal growth fac­tor (HB-EGF)-like growth fac­tor (EGF) is found in cere­bral neu­rons. Its expres­sion is increased after hypox­ic or ischemic injury, which also stim­u­lates neu­ro­ge­n­e­sis. HB-EGF has been impli­cat­ed as a par­tic­i­pant in a vari­ety of nor­mal phys­i­o­log­i­cal process­es such as blas­to­cyst implan­ta­tion and wound heal­ing, and in patho­log­i­cal process­es such as tumor growth, SMC hyper­pla­sia and ath­er­o­scle­ro­sis. HB-EGF is an 87 amino acid mito­genic and chemo­tac­tic gly­co­pro­tein con­tain­ing an EGF-like domain with six con­served cys­teine residues. Rat HB-EGF shares about 76 % a.a. sequence iden­ti­ty with human HB-EGF.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 9.7 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 86 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by a cell pro­lif­er­a­tion assay using murine Balb/​c 3T3 cells is less than 1 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 1.0 × 106 IU/​mg.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in PBS, 300 mM NaCl, pH 7.4, 5 % tre­halose.

Endo­tox­in

Less than 1 EU/​ug of rRtHB-EGF as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 95 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

sds page 145 08 2 - Recombinant Rat Heparin-binding EGF-like Growth Factor (rRtHB-EGF) CAS 145-08-1816

Pack Size

10ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Rat Heparin-bind­ing EGF-like Growth Fac­tor (rRtHB-EGF) on ChemWhat

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