Recombinant Rat Heparin-binding EGF-like Growth Factor (rRtHB-EGF) CAS 145-08-1816 Watson International Limited

Official Full Name

Recombinant Rat Heparin-binding EGF-like Growth Factor (rRtHB-EGF)

CAS Number (Or Watson Number for Non-CAS Products)

145-08-1816

Synonyms

HBEGF, DT-R

Squence

region 145 08 1 - Recombinant Rat Heparin-binding EGF-like Growth Factor (rRtHB-EGF) CAS 145-08-1816

Amino Acid Sequence

DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL

Synonyms

HBEGF, DT-R

Accession

Q06175

Gene ID

25433

Summary

Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Rat HB-EGF shares about 76 % a.a. sequence identity with human HB-EGF.

Source

Escherichia coli.

Molecular Weight

Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose.

Endotoxin

Less than 1 EU/ug of rRtHB-EGF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.