Overview

Gen­er­al Description

Recom­bi­nant SARS-COV-2 Spike S (Omi­cron B.1.1.529) Trimer Pro­tein is expressed from HEK293 with His tag at the C​-ter​mi​nal​.It con­tains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Pro­line sub­sti­tu­tions (F817P, A892P, A899P, A942P, K986P, V987P) and ala­nine sub­sti­tu­tions (R683A and R685A) are intro­duced to sta­bi­lize the trimer­ic pre­fu­sion state of SARS-CoV-2 S pro­tein and abol­ish the furin cleav­age site, respectively.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

Spike S (Omi­cron B.1.1.529) Trimer

Syn­onyms

S pro­tein ; Spike gly­co­pro­tein ; S gly­co­pro­tein ; COVID-19

Acces­sion

QHO60594

Species

SARS-COV-2

Exact Sequence

Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 136.70 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 140-180 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized SARS-COV-2 Spike S (Omi­cron B.1.1.529) Trimer, His Tag at 1ug/​ml (100ul/​well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 16.7ng/ml deter­mined by ELISA. See test­ing image for detail.

Prod­uct Tag

C-His

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 8% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Immo­bi­lized SARS-COV-2 Spike S (Omi­cron B.1.1.529) Trimer, His Tag at 1ug/​ml (100ul/​well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 16.7ng/ml deter­mined by ELISA.

SARS-COV-2 Spike S (Omi­cron B.1.1.529) Trimer on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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