Ultra­Nu­cle­ase CAS 9025-65-4

• Iden­ti­fi­ca­tion
• Prop­er­ties
• Safe­ty Data
• Spec­i­fi­ca­tions & Oth­er Information
• Links
• Quick Inquiry

Iden­ti­fi­ca­tion

CAS Number

9025-65-4

EC Number

3.1.30.2

Name

Ultra­Nu­cle­ase

Syn­onyms

1,4-Dihydro-1-ethyl-7-methyl-1,8-naphthyridin-4-one-3-carboxylic acid
1,8-Naphthyridine-3-carboxylic acid, 1-ethyl-1,4-dihydro-7-methyl-4-oxo- [ACD/​Index Name]
1450
1-Aethyl-7-methyl-1,8-naphthyridin-4-on-3-karbonsaeure [Ger­man]
1-Ethyl-1,4-dihydro-7-methyl-4-oxo-1,8-naphthyridine-3-carboxylic acid
1-Ethyl-7-methyl-4-oxo-1,4-dihydro-1,8-naphthyridin-3-carbonsäure [Ger­man] [ACD/IUPAC Name]
1-Ethyl-7-methyl-4-oxo-1,4-dihydro-1,8-naphthyridine-3-carboxylic acid [ACD/IUPAC Name]
222-159-7 [EINECS]
389-08-2 [RN]
Acide 1-éthyl-7-méthyl-4-oxo-1,4-dihydro-1,8-naphtyridine-3-carboxylique [French] [ACD/IUPAC Name]
Acide 1-etil-7-metil-1,8-naftiridin-4-one-3-carbossilico [Ital­ian]
Acide nalidix­i­co [Ital­ian]
acide nalidix­ique [French] [INN]
Aci­do nalidix­i­co [Span­ish] [INN]
áci­do nalidíx­i­co [Span­ish] [INN]
Acidum nalidix­i­cum [Latin] [INN]
Ethyl-7-methyl-1,8-naphthyridin-4-one-3-carboxylic Acid
nalidix­ic acid [INN] [JAN] [USAN] [Wiki]
Neg­Gram [Trade name]
QN2885000
SYSU-22645
T66 BV EN GNJ CVQ E2 H1 [WLN]
WIN-18320
WIN­TO­MY­LON
налидиксовая кислота [Russ­ian] [INN]
حمض ناليديكسيك [Ara­bic] [INN]
萘啶酸 [Chi­nese] [INN]
1, 4-Dihydro-1-ethyl-7-methyl-4-oxo-1,8-naphthyridine-3-carboxylic acid
1,4-Dihydro-1-ethyl-7-methyl-4-oxo-1,8-naphthyridine-3-carboxylic acid
1,8-Naphthyridine-3-carboxylicacid, 1-ethyl-1,4-dihydro-7-methyl-4-oxo-
1189467-36-4 [RN]
1-Aethyl-7-methyl-1,8-naphthyridin-4-on-3-karbonsaeure [Ger­man]
1-ethyl-1,4-dihydro-7-methyl-4-oxo-1,8-napthyridine-3-carboxylic acid
1-ethyl-4-keto-7-methyl-1,8-naphthyridine-3-carboxylic acid
1-Ethyl-7-methyl-1,4-dihydro-1, 8-naph­thyridin-4-one-3-car­boxylic acid
1-Ethyl-7-methyl-1,4-dihydro-1,8-naphthyridin-4-one-3-carboxylic acid
1-Eth­yl-7-methyl-4-oxo-1, 4-dihydro-1,8-naphthyridine-3-carboxylic acid
1-ethyl-7-methyl-4-oxo-1,4-dihydro-[1,8]naphthyrid
1-Ethyl-7-methyl-4-oxo-1,4-dihydro-[1,8]naphthyridine-3-carboxylic acid
1-ethyl-7-methyl-4-oxo-1,8-naphthyridine-3-carboxylic acid
1-ethyl-7-methyl-4-oxohydropyridino[2,3-b]pyridine-3-carboxylic acid
389-08-2,
3-Carboxy-1-ethyl-7-methyl-1,8-naphthidin-4-one
3-Carboxy-1-ethyl-7-methyl-1,8-naphthyridin-4-one
5-25-07-00384 (Beil­stein Hand­book Ref­er­ence) [Beil­stein]
75015
8/2/389
9025-49-4 [RN]
9025-65-4 [RN]
Acide 1-etil-7-metil-1,8-naftiridin-4-one-3-carbossilico
Acide 1-etil-7-metil-1,8-naftiridin-4-one-3-carbossilico [Ital­ian]
Acide nalidix­i­co [Ital­ian]
Acide nalidix­ique [French]
Acide nalidix­ique [INN-French]
acide nalidix­ique ; aci­do nalidix­i­co ; acidum nalidix­i­cum ; nalidix­ic acid
Acide nalidix­ique ; Aci­do nalidix­i­co ; Acidum Nalidix­i­cum ; Nalidixins??ure
Acide nalidix­ique ; Aci­do nalidix­i­co ; Acidum Nalidix­i­cum ; Nalidix­in­säure
Aci­do nalidis­si­co [DCIT]
aci­do nalidix­i­co
áci­do nalidíx­i­co
Aci­do nalidix­i­co [INN-Span­ish]
Acidum nalidix­i­cum [INN-Latin]
Adix
Adix (Trade name)
Adix ; Curiemy­lon ; Degram ; Del­u­gi ; Dix­i­con ; Glane­ga ; Gra­mazine ; Gra­moneg ; Huei Yi ; Lisalenb ; Litalon ; Nadix­in ; Nadon ; Nagomin ; Nal-acid ; Nal­ic ; Nali­cid ; Nalid ; Nalidin ; Nalidix ; Naligram ; Nal­it­o­my­lon ; Nal­ix ; Nal­ix­id ; Nal­ix­in ; Nebac­til ; Nega­chine ; Nevi­gra­mon ; Unaserus ; Win­to­my­lon ; Win­torin ; Youdix ; Zuno-Nathasid
Ben­zoic acid, 4-(aminomethyl)- (9CI)
Betax­i­na
BPBio1_​000125
BSPBio_​000113
BSPBio_​001889
Curiemy­lon
Curiemy­lon (Trade name)
Cybis
DB00779
Degram
Dix­i­nal
EINECS 206-864-7
eucis­ten
eucistin
Glane­ga
https://​www​.ebi​.ac​.uk/​c​h​e​b​i​/​s​e​a​r​c​h​I​d​.​d​o​?​c​h​e​b​i​I​d​=​C​H​E​B​I​:​1​0​0​147
Huei Yi
Huei Yi (Trade name)
IDI1_000058
Innox­alomn
Innox­alon
Jic­sron
Kus­nar­in
Lisalenb
Lopac0_​000837
N-1200
Nacid
Nadix­in
Nalidix­an
NALIDIX­ANE
Nalidix­anum
Nalidix­ic acid (JP15/USP/INN)
Nalidix­ic acid 100 µg/mL in Ace­toni­trile
nalidix­ic acid stan­dard
nalidix­ic acid, 99%
nalidix­ic acid, 99.5%
Nalidix­ic acid, Antibi­ot­ic for Cul­ture Media Use Only
NALIDIX­IC ACID-D5
nalidix­i­cacid
nalidix­in
Nalidixins??ure
Nalidixinsäure
Nalidix­in­säure
Naligram
Naligram
Nal­it­o­my­lon
Nal­i­tuc­san
N-Benzyl-4-[(5-cyclobutyl-1,2,4-oxadiazol-3-yl)methyl]-N-ethyl-3-oxo-3,4-dihydro-2H-1,4-benzoxazine-6-sulfonamide [ACD/IUPAC Name]
Nebac­til

SMILES

CCn1cc(c(=O)c2c1nc(cc2)C)C(=O)O

Std­InChI

InChI=1S/C12H12N2O3/c1-3-14-6-9(12(16)17)10(15)8-5-4-7(2)13-11(8)14/h4-6H,3H2,1-2H3,(H,16,17)

Std­InChIKey

MHWL­WQUZ­ZR­M­NGJ-UHF­F­­FAOYSA-N

Mol­e­c­u­lar Formula

C₁₂H₁₂N₂O₃

Mol­e­c­u­lar Weight

232.235

EINECS

222-159-7

MDL Number

MFCD00131010

Prop­er­ties

Appear­ance

Col­or­less liquid

Safe­ty Data

WGK Germany

2

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Ultra­Nu­cle­ase CAS 9025-65-4

Activ­i­ty Definition

At 37°C and pH 8.0, the amount of enzyme that reduces the △A260 val­ue by 1.0 (equiv­a­lent to com­plete diges­tion of 37 μg DNA) with­in 30 min­utes is defined as an activ­i­ty unit.

Iden­ti­fi­ca­tion Methods

SDS-PAGE ; SEC-HPLC

Puri­ty

99% min

pH

8.0

Pro­tein Spe­cif­ic Activity

≥1.0×10⁶ U/​mg

Iso­elec­tric Point

6.85

Opti­mum Temperature

37℃

Cofac­tor

1-10mM Mg²⁺

Stor­age Buffer

20mM pH 8.0 Tris-HCl, 2mM MgCl₂, 20mM NaCl, 50% (V/V) Glycerol

Pack­age

5KU,25KU,50KU,100KU,1MU,5MU

Shelf Life

2 years

Stor­age and Shelf Life 

Store at -20℃, avoid freez­ing and thaw­ing, and the vital­i­ty should not be less than 90% after 2 years of storage

Advan­tage

Ultra­Nu­cle­ase has high puri­ty, low host pro­tein residue, no ani­mal ori­gin, and sta­ble mass production.

Known Appli­ca­tion

Ultra­Nu­cle­ase is a broad-spec­trum nucle­ase, also known as a ver­sa­tile nucle­ase, which is a genet­i­cal­ly engi­neered nucle­ase derived from Ser­ra­tia Marcescens. It can degrade all forms of DNA and RNA, includ­ing sin­­gle-strand­ed, dou­ble-strand­ed, lin­ear, cir­cu­lar, nat­ur­al and dena­tured nucle­ic acids, and digest them into 5’monophosphate oligonu­cleotides with a length of 3-8 bases. It has no speci­fici­ty for base recog­ni­tion, and its hydrol­y­sis speed is 34 times that of DNase I and 4 times that of Staphy­lo­coc­cus aureus nucle­ase. It is the first choice for nucle­ic acid removal enzyme prepa­ra­tions in the bio­log­i­cal industry.

Enzy­mat­ic Prop­er­ties and Rec­om­mend­ed Con­di­tions Of Use

Items	Opti­mal Reac­tion Conditions	Effec­tive Reac­tion Conditions	Response Spec­trum
Tem­per­a­ture	37℃	0-45℃
pH	8.0 – 9.0	6.0 – 11.0
Mg2+	1 – 2mM	1 – 15mM
DTT	0 – 100mM	>100mM
2-Mer­­cap­­toethanol	0 – 100mM	>100mM
Na+、K+	0 – 20mM	0 – 200mM
PO43-	0 – 10mM	0 – 100mM

Ultra­Nu­cle­ase Clear

Anion Exchange Chromatography	Tan­gen­tial Flow Fil­tra­tion(TFF)
1. Rec­om­mend DEAE and TMAE chro­matog­ra­phy columns ; 2. The iso­elec­tric point of Almighty Nucle­ase is 6.85, which can’t be adsorbed.	1. A 300 kD mem­brane pack­age is rec­om­mend­ed, and the mol­e­c­u­lar weight of Almighty Nucle­ase is 29 kD ; 2. Tak­ing con­cen­trat­ed virus vac­cine as an exam­ple, the clear­ance rate of ultra­fil­tra­tion 5 vol­umes of Almighty Nucle­ase reach­es 99.5%.

Ultra­Nu­cle­ase Residue Detection

Ultra­Nu­cle­ase ELISA Kit (Altipo­tent Nucle­ase Residue Detec­tion Kit) is a high­ly sen­si­tive and high­ly spe­cif­ic kit for detect­ing and quan­ti­fy­ing the resid­ual pluripo­tent nucle­ase impu­ri­ties in recom­bi­nant virus vec­tors and vac­cine prepa­ra­tions. The detec­tion lim­it of the kit is 0.2 ng/​mL.

The stan­dard curve is not affect­ed by large intes­tine host pro­tein, yeast host pro­tein, mouse serum pro­tein, BSA and oth­er mis­cel­la­neous protein.

Links

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd (https://​www​.wat​son​-bio​.com/).

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