Identification
CAS Number
56941-23-2
EC Number
2.7.7.50
Name
Vaccinia Capping Enzyme
Properties
Optimal reaction temperature
37℃
Definition of active unit
Definition of active unit : 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α32P) GTP into 80nt transcript within 1h at 37℃ .
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our Vaccinia Capping Enzyme CAS 56941-23-2
Product Introduction
This product is the Vaccinia Capping Enzyme recombinantly expressed in E. coli. Vaccinia Capping Enzyme can add the 7-methylguanosine cap (Cap0) to the 5’ terminus of mRNA. This structure can improve the stability of mRNA, and is indispensable to subsequent transport and translation.
Product Features
Capping rate as high as 95%; the 5′-Cap structure effectively promoting the in vivo expression of mRNA.
Product Composition
| Item No. | Composition | Storage Temperature (℃ ) | 400U | 4000U |
| I | Vaccinia Capping Enzyme (10 U/μL)) | -20 | 40μL | 400μL |
| Ⅱ | Capping Buffer | -20 | 100μL | 1000μL |
| Ⅲ | S-adENosylmethionine (SAM 32mM) | -20 | 100μL | 1000μL |
| Ⅳ | GTP | -20 | 50μL | 500μL |
10× Capping Buffer contains : 0.5M Tris-HCl(pH8.0),50mM KCl,10mM MgCl2 and 10mM DTT.
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.Quality Control
Links
This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).
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