Iden­ti­fi­ca­tion

CAS Number

9025-42-7

EC Number

3.2.1.24

Name

α-Man­nosi­­dase CAS 9025-42-7

Syn­onyms

ALPHA-MAN­NOSI­­DASE ; ALPHA-D-MAN­NOSIDE­­MAN­NO­HY­­DRO­LASE ; ALPHA1,6-MAN­NOSI­DASE ; ALPHA1-2,3-MAN­NOSI­DASE ; EC3.2.1.24 ; a1,2Mannoisdase,Trichodermareesei,Recomb.,Saccharomyces ; a1,6-Mannosidase,Xanthomonassp.,Recomb.,E.coli ; a1-2,3-Mannosidase,Recomb.,E.coli

Prop­er­ties

Phys­i­cal form

Sus­pen­sion in 50 mM PB buffer, pH 6.5

Mol­e­c­u­lar weight

220 kDa (SDS-PAGE)

Michaelis con­stant

5.2 ×10-4mol/L (p-nitro­phenyl α-D-mannopyranoside)

Opti­mum pH

6.0

Opti­mum temperature

70 ℃

pH Sta­bil­i­ty

5~7 (25 ℃, 24 hrs )

Ther­mal stability

60 ℃ (pH 6.0, 24hrs)

Inhibitors

EDTA, swain­so­nine

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our α-Man­nosi­­dase CAS 9025-42-7

Unit def­i­n­i­tion

One unit of activ­i­ty is defined as the amount of enzyme that released 1 μmol of p-nitro­phe­nol per min at pH 6.0 and 60°C using p-nitro­phenyl α-D-mannopy­­ra­no­side as a substrate.

Appli­ca­tion

α-Man­nosi­­dase can be used to lib­er­ate man­nose from a vari­ety of syn­thet­ic and nat­ur­al α-man­no­­sides and cleaves off α-1,2-, α-1,3-, α-1,4-, and α-1,6-linked man­nose residues.

Spe­cif­ic activity

≥30 U/​mg protein

Shelf Life

2 years at 4°C

Stor­age

Store at 2-8°C for long time, Sealed and away from light

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd (https://​www​.wat​son​-bio​.com/).

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