Identification
CAS Number
9027-73-0
Name
5’-Nucleotidase
Synonyms
5′-NUCLEOTIDASE FROM CROTALUS ATROX VENOM;
5′-nucleotidase from crotalus adamanteus venom;
Nucleotidase, 5′-;5′-NT;5′-Nucleotidase human ;
CD73;
ecto-5′-nucleotidase;
NT5E
Molecular Formula
232-820-1
Molecular Weight
447.225
MDL Number
MFCD00130311
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our
5’-Nucleotidase CAS 9027-73-0
General Assay Characteristics
| Method | Enzymatic/RATE A/E |
| Reagents | Ready to use |
| Calibrators | Level 1-0.0 U/L; Level 2-60 U/L |
| Calibration Stability | 28 days |
| Sample Type | EDTA,SST,Serum,Heparinised plasma |
| Assay Range | 1-100U/L undiluted |
| Standardisation |
Assay Principle
Proposed 5’-NT Recipe
Reagent I
| Composition | Concentration | Catalog Number |
| Tris-HCl buffer | 50mM | |
| Xanthine Oxidase | ＞1KU/L | EM022 |
| 4-Aminoantipyrine | 1mM | |
| Purine Nucleoside | ＞1KU/L | EM021 |
| Phosphorylase | ||
| POD | ＞1KU/L | |
| Detergent, Preservative |
Reagent II
| Composition | Concentration | Catalog Number |
| Tris-HCl buffer | 50mM | |
| 5’-IMP | 10mM | |
| TOOS | 10mM | |
| EDTA | 8mM | |
| Detergent, Preservative |
Major reagent ingredients made by Assay Specilist Lab are list on right
Standard&Control
Home Made: 5’-NT(CC001) in buffer
Reaction curve chart
Known Application
Commonly used in biochemical research, food preservation, clinically used as a diagnostic enzyme, determination of phosphoric acid and creatine phosphate.
