Iden­ti­fi­ca­tion

CAS Number

9027-73-0

Name

5’-Nucleotidase

Syn­onyms

5′-NUCLEOTIDASE FROM CRO­TALUS ATROX VEN­OM ;
5′-nucleotidase from cro­talus adaman­teus ven­om ;
Nucleoti­dase, 5′-;5′-NT;5′-Nucleotidase human ;
CD73 ;
ecto-5′-nucleotidase ;
NT5E

Mol­e­c­u­lar Formula

232-820-1

Mol­e­c­u­lar Weight

447.225 

MDL Number 

MFCD00130311

Safe­ty Data

RIDADR 

NONH for all modes of transport 

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our
5’-Nucleotidase CAS 9027-73-0

Gen­er­al Assay Characteristics 

Method
Enzymatic/​RATE A/E
ReagentsReady to use
Cal­i­bra­torsLev­el 1-0.0 U/L ; Lev­el 2-60 U/L
Cal­i­bra­tion Stability28 days
Sam­ple TypeEDTA,SST,Serum,Heparinised plas­ma
Assay Range1-100U/L undi­lut­ed
Stan­dard­i­s­a­tion

Assay Prin­ci­ple

Pro­posed 5’-NT Recipe 

Reagent I

Com­po­si­tionCon­cen­tra­tionCat­a­log Number
Tris-HCl buffer50mM
Xan­thine Oxidase＞1KU/LEM022
4-Aminoan­tipyrine1mM
Purine Nucle­o­side＞1KU/LEM021
Phos­pho­ry­lase
POD＞1KU/L
Deter­gent, Preservative

Reagent II

Com­po­si­tionCon­cen­tra­tionCat­a­log Number
Tris-HCl buffer50mM
5’-IMP10mM
TOOS10mM
EDTA8mM
Deter­gent, Preservative

Major reagent ingre­di­ents made by Assay Spe­cilist Lab are list on right

Stan­dard&Con­trol

Home Made : 5’-NT(CC001) in buffer

Reac­tion curve chart 

Known Appli­ca­tion

Com­mon­ly used in bio­chem­i­cal research, food preser­va­tion, clin­i­cal­ly used as a diag­nos­tic enzyme, deter­mi­na­tion of phos­phor­ic acid and cre­a­tine phosphate.

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd(http://​www​.ulcho​.com/), and here is the cor­re­spond­ing linkhttp://​www​.ulcho​.com/​5​-​n​u​c​l​e​o​t​i​d​a​s​e​-​c​a​s​-​9​0​2​7​-​7​3​-0/

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