Iden­ti­fi­ca­tion

CAS Number

UENA-0244

Name

Ascor­bate oxidase

Syn­onyms

ASCOR­BASE ;
ASCOR­BATE OXI­DASE ;
ASCOR­BATE OXI­DASE CUCUR­BI­TA SPECIES ;
EC 1.10.3.3 ;
L-ASCOR­­BATE : OXY­GEN OXI­DORE­DUC­TASE ;
Oxi­dase, ascor­bate ;
ASCOR­BATE OXI­DASE FROM CUCUR­BITASPECIES

Mol­e­c­u­lar weight

80 kDa (Gel filtration)

Prop­er­ties

Appear­ance

Light blue amor­phous pow­der, lyophilized

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Ascor­bate oxi­dase CAS UENA-0244

Activ­i­ty

≥100 U/​mg solid

Km

Ascor­bic acid (pH 7.0) 1.0×10-4 M
Ascor­bic acid (pH 4.0) 3.8×10-4 M

Opti­mum pH

4.0 – 4.5 (Acetate buffer)

Opti­mum temperature

55℃

pH sta­bil­i­ty

6.0 – 9.0 (30℃, 24 hr)

Ther­mal stability

Sta­ble at 50 ℃ and below (pH 7.0,10 min)

SOL. (50mg/​ml water)

Cor­re­sponds

PH (50mg/​ml water)

7.5

ACT. (25°C ; with L-ascorbate)

178 u/​mg L

SPEC. ACT. (25°C ; with L-ascorbate)

2119 u/​mg P

ACT. (37°C ; with L-ascorbate)

278 u/​rng L

SPEC. ACT. (37°C ; with L-ascorbate)

3471 u/​mg P

PRO­TEIN (BCA reag.)

0.084 mg/​mg L

Con­t­a­m­i­nat­ing Activities

Cor­re­sponds

GOT

<0.00005%

GPT

<0.00006%

Cata­lase

<0.008%

Con­t­a­m­i­nat­ing Oxidases

<0.00003%

Stor­age stability

-20 ℃ for 12 months for more than 90% activity.

Stor­age

Store at -20°C for long time, sealed and away from light.

Known Appli­ca­tion

This enzyme is main­ly used to effec­tive­ly pre­vent the inter­fer­ence of ascor­bic acid on diag­nos­tic tests such as blood, uric acid, TG, TC, and creatinine.

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd (http://​www​.ulcho​.com/).

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