- Overview
- Specifications
- Shipping, Reconstitution & Storage
- General View of Documents
- Declaration
- Quick Inquiry
Overview
General Description
Recombinant Biotinylated Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Val35-Asn242.
Biotinylated
Yes
Specifications
Target
B7-1/CD80
Synonyms
CD80 molecule ; B7 ; B71 ; B7-1 ; BB1 ; CD28LG1 ; CD80 ; B7.1 ; CD28LG1 ; LAB7
Accession
Species
Human
Exact Sequence
Val35-Asn242
Molecular Weight
The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Human CTLA-4, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-1, His Tag with the EC50 of 1.9ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Human CTLA-4, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-1, His Tag with the EC50 of 1.9ug/ml determined by ELISA.
The purity of Biotinylated Human B7-1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
Quick Inquiry
Fill out our inquiry form and one of our experts will be in touch with you shortly.