Official Full Name
Biotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi (rHuMIF,His,Avi,Biotinylated)
CAS Number (Or Watson Number for Non-CAS Products)
601-033-1816
Synonyms
Squence
Amino Acid Sequence
Synonyms
Accession
Gene ID
Summary
Migration Inhibitory Factor (
MIF) is a secreted protein without a cleavable signal sequence and is secreted via a specialized, nonclassicalpathway. It is secreted by macrophages upon stimulation by bacterial lipopolysaccharide (
LPS), or by M.tuberculosis antigens.
MIF consists of two alpha-helices and six beta-strands, four of which form a beta-sheet. The two remaining beta-strands interact with other
MIF molecules, creating a trimer. Structure-function studies suggest
MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position 1. Amino acids 50-65(a.a.) have also been suggested to contain thiol-protein oxidoreductase activity.
MIF has proinflammatory cytokine activity centered around 49 – 65(a.a.). On fibroblasts,
MIF induces,
IL-1,
IL-8 and
MMP expression ; on macrophages,
MIF stimulates
NO production and TNF-alpha release folllowing IFN-gamma activation.
MIF apparently acts through
CD74 and
CD44, likely in some form of trimeric interaction. Human
MIF is active on mouse cells. Human
MIF is 90 %, 94 %, 95 %, and 90 % a.a. identical to mouse, bovine, porcine and rat
MIF, respectively.
Source
Escherichia coli.
Molecular Weight
Biological Activity
Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional
ELISA.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in
PBS, pH 7.0, 5 % Trehalose, 0.02 % Tween-20.
Endotoxin
Less than 0.1
EU/ug of rHuMIF, His, Avi, Biotinylated as determined by
LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 %
BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
Purity
> 97 % by
SDS-PAGE and
HPLC analyses.
SDS-PAGE
Pack Size
10ug/100ug/500ug
Safety Data Sheet (SDS) Download
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Technical Data Sheet (TDS) Download
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Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link
Biotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi (rHuMIF,His,Avi,Biotinylated) on ChemWhat
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