Official Full Name
Biotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi (rHuMIF,His,Avi,Biotinylated)
CAS Number (Or Watson Number for Non-CAS Products)
Amino Acid Sequence
Migration Inhibitory Factor (MIF
) is a secreted protein without a cleavable signal sequence and is secreted via a specialized, nonclassicalpathway. It is secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS
), or by M.tuberculosis antigens. MIF
consists of two alpha-helices and six beta-strands, four of which form a beta-sheet. The two remaining beta-strands interact with other MIF
molecules, creating a trimer. Structure-function studies suggest MIF
is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position 1. Amino acids 50-65(a.a.) have also been suggested to contain thiol-protein oxidoreductase activity. MIF
has proinflammatory cytokine activity centered around 49 – 65(a.a.). On fibroblasts, MIF
expression ; on macrophages, MIF
production and TNF-alpha release folllowing IFN-gamma activation. MIF
apparently acts through CD74
, likely in some form of trimeric interaction. Human MIF
is active on mouse cells. Human MIF
is 90 %, 94 %, 95 %, and 90 % a.a. identical to mouse, bovine, porcine and rat MIF
Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered concentrated solution in PBS
, pH 7.0, 5 % Trehalose, 0.02 % Tween-20.
Less than 0.1 EU
/ug of rHuMIF, His, Avi, Biotinylated as determined by LAL
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA
to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
> 97 % by SDS-PAGE
Safety Data Sheet (SDS) Download
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Technical Data Sheet (TDS) Download
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