Official Full Name

Biotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi (rHuMIF,His,Avi,Biotinylated)

CAS Number (Or Watson Number for Non-CAS Products)

601-033-1816

Synonyms

Squence

Squence of Biotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi

Amino Acid Sequence

Synonyms

Accession

Gene ID

Summary

Migration Inhibitory Factor (MIF) is a secreted protein without a cleavable signal sequence and is secreted via a specialized, nonclassicalpathway. It is secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. MIF consists of two alpha-helices and six beta-strands, four of which form a beta-sheet. The two remaining beta-strands interact with other MIF molecules, creating a trimer. Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position 1. Amino acids 50-65(a.a.) have also been suggested to contain thiol-protein oxidoreductase activity. MIF has proinflammatory cytokine activity centered around 49 – 65(a.a.). On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release folllowing IFN-gamma activation. MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction. Human MIF is active on mouse cells. Human MIF is 90 %, 94 %, 95 %, and 90 % a.a. identical to mouse, bovine, porcine and rat MIF, respectively.

Source

Escherichia coli.

Molecular Weight

Biological Activity

Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0, 5 % Trehalose, 0.02 % Tween-20.

Endotoxin

Less than 0.1 EU/ug of rHuMIF, His, Avi, Biotinylated as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Purity

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE

SDS-PAGE of Biotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi

Pack Size

10ug/100ug/500ug

Safety Data Sheet (SDS) Download

Click to download

Technical Data Sheet (TDS) Download

Click to download

Links

This product is branded by ChemWhat and sold through our website and here is the corresponding link Biotinylated Recombinant Human Macrophage Migration Inhibitory Factor, His, Avi (rHuMIF,His,Avi,Biotinylated) on ChemWhat

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