Identification
CAS Number
80498-17-5
EC Number
3.1.21.4
Name
EcoR I
Synonyms
EcoR I from Escherichia coli BS5
Restriction Enzyme
EINECS
279-487-9
MDL Number
MFCD00163532
Properties
Optimal reaction temperature
37℃
Definition of active unit
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to degrade 1μgλ of DNA within 1h at 37℃ in a 50μL reaction system.
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our EcoR I CAS 80498-17-5
Product Introduction
This product is the EcoRI capable of recognizing the G^AATTC site recombinantly expressed in E. coli. EcoRI is widely used in fields including gene mapping and cloning. It can cut DNA rapidly, to achieve gene linearization of high efficiency.
Product Features
High enzyme activity; rapid completion of cleavage reaction.
Low non-specific endonuclease activity, to ensure “scalpel-like” precise cutting.
No BSA system, less heat source contamination.
Product Composition
| Item No. | Composition | Storage Temperature (℃ ) | 10KU | 100KU |
| I | EcoRI (20 U/μL) | -20 | 0.5mL | 5mL |
| Ⅱ | 10x Buffer | -20 | 1.25mL | 12.5mL |
Quality Control
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Links
This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).
Quick Inquiry
Fill out our inquiry form and one of our experts will be in touch with you shortly.
