Structue of EcoR I CAS 80498 17 5 600x268 - EcoR I CAS 80498-17-5

Iden­ti­fi­ca­tion

CAS Number

80498-17-5

EC Number

3.1.21.4

Name

EcoR I

Syn­onyms

EcoR I from Escherichia coli BS5
Restric­tion Enzyme

EINECS

279-487-9

MDL Number

MFCD00163532

Prop­er­ties

Opti­mal reac­tion temperature

37℃

Def­i­n­i­tion of active unit

Def­i­n­i­tion of active unit : 1 active unit is defined as the amount of enzyme need­ed to degrade 1μgλ of DNA with­in 1h at 37℃ in a 50μL reac­tion system.

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our EcoR I CAS 80498-17-5

Prod­uct Introduction

This prod­uct is the EcoRI capa­ble of rec­og­niz­ing the G^AATTC site recom­bi­nant­ly expressed in E. coli. EcoRI is wide­ly used in fields includ­ing gene map­ping and cloning. It can cut DNA rapid­ly, to achieve gene lin­eariza­tion of high efficiency.

Prod­uct Features

High enzyme activ­i­ty ; rapid com­ple­tion of cleav­age reac­tion.
Low non-spe­­cif­ic endonu­cle­ase activ­i­ty, to ensure scalpel-like” pre­cise cut­ting.
No BSA sys­tem, less heat source contamination.

Prod­uct Composition

Item No.Com­po­si­tionStor­age Tem­per­a­ture
(℃ )
10KU100KU
IEcoRI (20 U/​μL)-200.5mL5mL
10x Buffer-20 1.25mL12.5mL

Qual­i­ty Control

Puri­ty ≥ 95%, resid­ual host cell DNA ≤ 100pg/​mg, resid­ual host cell pro­tein ≤ 50ppm, resid­ual endo­tox­in ≤10EU/​mg, no resid­ual RNase, endonu­cle­ase, exonu­cle­ase or pro­tease, germ-free, pathogen-free.

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd (http://​www​.ulcho​.com/).

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