Structure of Fibroblast Growth Factor CAS 62031 54 3 - Fibroblast Growth Factor CAS 62031-54-3

custom peptides - Fibroblast Growth Factor CAS 62031-54-3

Iden­ti­fi­ca­tion

CAS Number

62031-54-3

Name

Fibrob­last Growth Factor/​rh BFGF

Sequence

H-Lys-Arg-Thr-Gly-Gln-Tyr-Lys-Leu-OH

Syn­onyms

L-Pro­­lyl-L-alanyl-L-leucyl-L-pro­­lyl-L-α-glu­­tamyl-L-α-asparagyl­g­ly­­cyl­g­ly­­cyl-L-seryl­g­ly­­cyl-L-alanyl-L-pheny­lalanyl-L-pro­­lyl-L-pro­­lyl­g­ly­­cyl-L-his­tidyl-L-pheny­lalanyl-L-lysyl-L-α-asparagyl-L-p rolyl-L-lysyl-L-arginyl-L-leucyl-L-tyrosin [Ger­man] [ACD/IUPAC Name]
L-Pro­­lyl-L-alanyl-L-leucyl-L-pro­­lyl-L-α-glu­­tamyl-L-α-aspartyl­g­ly­­cyl­g­ly­­cyl-L-seryl­g­ly­­cyl-L-alanyl-L-pheny­lalanyl-L-pro­­lyl-L-pro­­lyl­g­ly­­cyl-L-his­tidyl-L-pheny­lalanyl-L-lysyl-L-α-aspartyl-L-pro lyl-L-lysyl-L-arginyl-L-leucyl-L-tyro­­sine [ACD/IUPAC Name]
L-Pro­­lyl-L-alanyl-L-leucyl-L-pro­­lyl-L-α-glu­­tamyl-L-α-aspartyl­g­ly­­cyl­g­ly­­cyl-L-séryl­g­ly­­cyl-L-alanyl-L-phény­lalanyl-L-pro­­lyl-L-pro­­lyl­g­ly­­cyl-L-his­tidyl-L-phény­lalanyl-L-lysyl-L-α-aspartyl-L-pro lyl-L-lysyl-L-arginyl-L-leucyl-L-tyro­­sine [French] [ACD/IUPAC Name]
L-Tyro­­sine, L-pro­­lyl-L-alanyl-L-leucyl-L-pro­­lyl-L-α-glu­­tamyl-L-α-aspartyl­g­ly­­cyl­g­ly­­cyl-L-seryl­g­ly­­cyl-L-alanyl-L-pheny­lalanyl-L-pro­­lyl-L-pro­­lyl­g­ly­­cyl-L-his­tidyl-L-pheny­lalanyl-L-lysyl-L-α-as partyl-L-pro­­lyl-L-lysyl-L-arginyl-L-leucyl- [ACD/​Index Name]
211362-85-5 [RN]
62031-54-3 [RN]
MFCD00133317

SMILES

C[C@@H](C(=O)N[C@@H](Cc1ccccc1)C(=O)N2CCC[C@H]2C(=O)N3CCC[C@H]3C(=O)NCC(=O)N[C@@H](Cc4c[nH]cn4)C(=O)N[C@@H](Cc5ccccc5)C(=O)N[C@@H](CCC­CN)C(=O)N[C@@H](CC(=O)O)C(=O)N6CCC[C@H]6C(=O)N[C@@H](CCC­CN)C(=O)N[C@@H](CCC­NC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc7ccc(cc7)O)C(=O)O)NC(=O)CNC(=O)[C@H](CO)NC(=O)CNC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H]8CCCN8C(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]9CCCN9

Std­InChI

InChI=1S/C118H173N31O33/c1-64(2)49-78(107(171)145-85(117(181)182)53-70-35-37-72(151)38-36-70)139-104(168)76(30-18-44-125-118(121)122)135-103(167)75(28-14-16-42-120)137-111(175)88-32-21-46-147(88)115(179)84(56-97(160)161)144-105(169)74(27-13-15-41-119)136-108(172)79(51-68-23-9-7-10-24-68)140-109(173)80(54-71-57-123-63-130-71)133-94(155)61-129-110(174)87-31-19-47-148(87)116(180)90-34-22-48-149(90)114(178)83(52-69-25-11-8-12-26-69)143-98(162)66(5)131-92(153)60-128-101(165)86(62-150)134-93(154)59-126-91(152)58-127-100(164)81(55-96(158)159)141-106(170)77(39-40-95(156)157)138-112(176)89-33-20-45-146(89)113(177)82(50-65(3)4)142-99(163)67(6)132-102(166)73-29-17-43-124-73/h7-12,23-26,35-38,57,63-67,73-90,124,150-151H,13-22,27-34,39-56,58-62,119-120H2,1-6H3,(H,123,130)(H,126,152)(H,127,164)(H,128,165)(H,129,174)(H,131,153)(H,132,166)(H,133,155)(H,134,154)(H,135,167)(H,136,172)(H,137,175)(H,138,176)(H,139,168)(H,140,173)(H,141,170)(H,142,163)(H,143,162)(H,144,169)(H,145,171)(H,156,157)(H,158,159)(H,160,161)(H,181,182)(H4,121,122,125)/t66-,67-,73-,74-,75-,76-,77-,78-,79-,80-,81-,82-,83-,84-,85-,86-,87-,88-,89-,90-/m0/s1

Std­InChIKey

YSFTYXKQUON­N­­FY-NQX­PE­FQP­SA-N

Mol­e­c­u­lar Formula

C118H173N31O33

Mol­e­c­u­lar Weight

2553.824

MDL Number

MFCD00166724

Prop­er­ties

Appear­ance

White or off-white lyophilized powder

Safe­ty Data

Per­son­al Pro­tec­tive Equipment

dust mask type N95 (US), Eye­shields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Fibrob­last Growth Fac­tor CAS 62031-54-3

Descrip­tion

Recom­bi­nant human basic fibrob­last growth fac­tor (also known as basic FGF, bFGF, FGF2, FGF-β or heparin-bind­ing growth fac­tor) is a bio­log­i­cal­ly active pro­tein suit­able for cell cul­ture appli­ca­tions. The bFGF reg­u­lates var­i­ous process­es such as cell pro­lif­er­a­tion, dif­fer­en­ti­a­tion, sur­vival, adhe­sion, exer­cise, apop­to­sis, limb for­ma­tion and wound heal­ing. The bFGF can be used for angio­gen­e­sis, fibrob­last mito­sis, axon­al growth of PC-12 cells, recep­tor bind­ing and tyro­sine phos­pho­ry­la­tion stud­ies.
The prod­uct is lyophilized pow­der pre­pared by recom­bi­nant E.coli expres­sion, sep­a­rat­ed and puri­fied by mul­ti­ple steps, dis­solve in 10mM PBS, 0.15 M NaCl (pH7.2) solu­tion and then ster­il­ized by fil­tra­tion through a 0.22μm fil­ter mem­branes and freeze-dried.

Advan­tage

  1. No ani­mal ori­gin : no ani­mal dis­eases, no path­o­gen­ic sub­stances, no con­t­a­m­i­na­tion by exoge­nous fac­tors, and high safety.
  2. Qual­i­ty sta­bil­i­ty : This prod­uct is a lyophilized pow­der con­tain­ing a bio­log­i­cal­ly active pro­tec­tive agent. It has been mass-pro­­duced, and the dif­fer­ence between batch­es is small ; the pro­duc­tion scale is more than 1000L.

Prop­er­ties

SpeciesHuman
SourceE.coli
MW17.3KD±1.7kDa
Puri­ty≥95%(SDS-PAGE
Endo­tox­in< 0.2 EU/​µg
Asep­ticFil­ter and ster­il­ize with 0.22μm fil­ter before freeze-drying
Iso­elec­tric point9.5-9.6
Activ­i­ty≥5.0 x 106IU /​mg pro.
Sol­u­bil­i­tyGood sol­u­bil­i­ty in water, buffer, and medium

Bio­log­i­cal activity

The bio­log­i­cal activ­i­ty of the mouse fibrob­last cell line BALB/3T3 was deter­mined by the method of Chi­nese Phar­ma­copoeia 2020 edi­tion using bFGF.

Rec­om­mend­ed Method

It is rec­om­mend­ed cen­trifug­ing the pow­der to the bot­tom of the tube before open­ing the lid, then dis­solve it in ster­il­ized demo­nized water to pre­pare a stor­age solu­tion of 100 μg/​ml. It can be stored sta­bly for 2 weeks at 2℃ ~ 8℃.When dilut­ed to a low­er con­cen­tra­tion (not less than 10μg/​ml), 0.1% tis­sue cul­ture grade BSA or HSA is required. If it is nec­es­sary to extend the stor­age time, it is rec­om­mend­ed to freez­ing at -80℃ or -20℃ rather than multigelation.This prod­uct should be estab­lished by exper­i­ment to deter­mine the opti­mum con­cen­tra­tion accord­ing to each spe­cif­ic application.

Trans­port and Stor­age Stability

Trans­porta­tion sta­bil­i­ty : It is trans­port­ed in an ice pack to keep it active. 

Stor­age sta­bil­i­ty : sta­ble to 12 months at -15~-20°C and 24 months at -80°C, sta­ble to 4 weeks at 2~8℃. Pre­pare as a stor­age solu­tion of 100μg/​ml, sta­ble stor­age at 2~8℃ for one week.

Known Appli­ca­tion

Used in cell cul­ture to enhance cell pro­lif­er­a­tion, cell growth and cell differentiation.

This prod­uct is devel­oped by our R&D com­pa­ny Apnoke Sci­en­tif­ic Ltd (http://​www​.apnoke​.com/).

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