Overview

Gen­er­al Description

Recom­bi­nant Human BAFF/TNFSF13B/CD257 Pro­tein is expressed from HEK293 with hFc tag at the N​-ter​mi​nal​.It con­tains Ala134-Leu285.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

BAFF/TNFSF13B/CD257

Syn­onyms

BAFF ; BLyS ; CD257 ; TNFSF13B ; TNFSF20 ; DTL ; TALL1 ; TALL-1delta BAFF ; TALL1Delta4 BAFF ; THANK ; ZTNF4 ; TALL-1

Acces­sion

Q9Y275

Species

Human

Exact Sequence

Ala134-Leu285

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 41.7 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 45-48 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human BCMA Trimer, His Tag at 0.5ug/ml (100ul/​Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/​ml deter­mined by ELISA (QC Test). The affin­i­ty con­stant of 5.83 uM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

N-monomer­ic hFc

Express Sys­tem

HEK293

Puri­ty

> 90% as deter­mined by Tris-Bis PAGE;> 90% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 5% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Human BAFF, hFc Tag cap­tured on CM5 Chip via Pro­tein A can bind Mouse BCMA, His-Avi Tag with an affin­i­ty con­stant of 5.83 uM as deter­mined in SPR assay (Bia­core T200).

Immo­bi­lized Human BCMA Trimer, His Tag at 0.5ug/ml (100ul/​Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/​ml deter­mined by ELISA (QC Test).

The puri­ty of Human BAFF is greater than 95% as deter­mined by SEC-HPLC.

Human BAFF on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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