compound no - T7 RNA Polymerase CAS 9014-24-8

Iden­ti­fi­ca­tion

CAS Number

9014-24-8

EC Number

2.7.7.6

Name

T7 RNA Polymerase

EINECS

232-756-4

Prop­er­ties

Opti­mal reac­tion temperature

37℃

Def­i­n­i­tion of active unit

Def­i­n­i­tion of active unit : 1 active unit is defined as the amount of enzyme need­ed to incor­po­rate 1 nmol of [3H] GMP into acid-insol­u­ble pre­cip­i­tate with­in 1h under the con­di­tions of 37℃ and pH8.

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our T7 RNA Poly­merase CAS 9014-24-8

Prod­uct Introduction

This prod­uct is the phage T7 RNA Poly­merase derived from recom­bi­nant expres­sion in E. coli. It can specif­i­cal­ly rec­og­nize the T7 pro­mot­er sequence, and syn­the­size RNA com­ple­men­tary to down­stream sin­­gle-strand­ed DNA of the pro­mot­er using the sin­gle or dou­ble strand­ed DNA con­tain­ing the sequence of T7 pro­mot­er as the tem­plate and NTP as the substrate.

Prod­uct Features

Excel­lent RNA out­put rate ; homo­ge­neous tran­script length ; tran­script length up to 10K.

Product Features 3 - T7 RNA Polymerase CAS 9014-24-8
Prod­uct Features

Prod­uct Composition

Item No.Com­po­si­tionStor­age Tem­per­a­ture
(℃ )
5KU50KU
IT7RNA Poly­merase (50 U/​μL)-20100μL1000μL
Buffer-20 400mL4000mL

1× Buffer con­tains : 40 mM Tris HCl(pH7.9), 6mM MgCl2, 1mM DTT and 2mM sper­mi­dine, 10mM NaCl.

Qual­i­ty Control

Puri­ty ≥ 95%, resid­ual host cell DNA ≤ 100pg/​mg, resid­ual host cell pro­tein ≤ 50ppm, resid­ual endo­tox­in ≤10EU/​mg, no resid­ual RNase, endonu­cle­ase, exonu­cle­ase or pro­tease, germ-free, pathogen-free.

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd (http://​www​.ulcho​.com/).

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