Overview

Gen­er­al Description

Recom­bi­nant Human CD3E&CD3D/CD3 epsilon&CD3 delta Pro­tein is expressed from HEK293 with His tag at the C​-ter​mi​nal​.It con­tains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D).

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

CD3E&CD3D/CD3 epsilon&CD3 delta

Syn­onyms

CD3 ; CD3e ; CD3E ; CD3d ; T3D ; CD3D ; CD3E&CD3D ; CD3 delta&CD3 epsilon

Acces­sion

P07766(CD3E)&P04234(CD3D)

Species

Human

Exact Sequence

Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to gly­co­sy­la­tion, the pro­tein migrates to 28-38 kDa and 25 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human CD3E&CD3D at 2ug/​ml (100ul/​Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/​ml deter­mined by ELISA (QC Test). The affin­i­ty con­stant of 0.36nM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

C-His

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 5% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Human CD3E&CD3D, His Tag cap­tured on CM5 Chip via Anti-His Anti­body can bind OKT3, mFc Tag with an affin­i­ty con­stant of 0.36nM as deter­mined in SPR assay (Bia­core T200).

Immo­bi­lized Human CD3E&CD3D at 2ug/​ml (100ul/​Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/​ml deter­mined by ELISA (QC Test).

Immo­bi­lized Human CD3E&CD3D at 2ug/​ml (100ul/​Well). Dose response curve for Foralum­ab, hFc Tag with the EC50 of 18.5ng/ml deter­mined by ELISA.

The puri­ty of Human CD3E&CD3D was greater than 95% as deter­mined by SEC-HPLC.

Human CD3E&CD3D on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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