Overview

General Description

Recombinant Human CLEC9A Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Lys57-Val241.

Biotinylated

Specifications

Target

CLEC9A

Synonyms

CD370; CLEC9A; 9830005G06Rik; DNGR-1; HEEE9341; UNQ9341

Accession

Q6UXN8

Species

Human

Exact Sequence

Lys57-Val241

Molecular Weight

The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CLEC9A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

General View of Documents

Immobilized Human CLEC9A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

The purity of Human CLEC9A is greater than 95% as determined by SEC-HPLC.

Human CLEC9A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).

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