![Human ICOS/CD278 (Q50A) Protein, Accession: Q9Y6W8 20220304092051 600x400 - Human ICOS/CD278 (Q50A) Protein, Accession: Q9Y6W8](https://www.watson-int.com/wp-content/uploads/2023/07/20220304092051-600x400.jpg)
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Overview
General Description
Recombinant Human ICOS/CD278 (Q50A) Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Glu21-Phe141.
Biotinylated
Specifications
Target
ICOS/CD278 (Q50A)
Synonyms
AILIM ; CD278 ; CRP-1 ; CVID1 ; ICOS ; MGC39850
Accession
Species
Human
Exact Sequence
Glu21-Phe141
Molecular Weight
The protein has a predicted MW of 40.45 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity
The affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
![Human ICOS/CD278 (Q50A) Protein, Accession: Q9Y6W8 20220304092051 - Human ICOS/CD278 (Q50A) Protein, Accession: Q9Y6W8](/wp-content/uploads/validation-images/20220304092051.jpg)
Human ICOS (Q50A) , hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).
![Human ICOS/CD278 (Q50A) Protein, Accession: Q9Y6W8 20220228115755 - Human ICOS/CD278 (Q50A) Protein, Accession: Q9Y6W8](/wp-content/uploads/validation-images/20220228115755.jpg)
The purity of Human ICOS (Q50A) is greater than 95% as determined by SEC-HPLC.
![Human ICOS/CD278 (Q50A) Protein, Accession: Q9Y6W8 20220228115545 - Human ICOS/CD278 (Q50A) Protein, Accession: Q9Y6W8](/wp-content/uploads/validation-images/20220228115545.jpg)
Human ICOS (Q50A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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