- Overview
- Specifications
- Shipping, Reconstitution & Storage
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Overview
General Description
Recombinant Human IL-12 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Ile23-Ser328 (IL-12B) & Arg23-Ser219 (IL-12A).
Biotinylated
Specifications
Target
IL-12
Synonyms
CLMF ; CLMF2 ; IL-12A ; IL-12B ; IL12 ; IL12 p70 ; IMD28 ; IMD29 ; NFSK ; NKSF1 ; NKSF2 ; P35 ; IL-12 subunit p35 ; IL12A ; interleukin 12
Accession
Species
Human
Exact Sequence
Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)
Molecular Weight
The protein has a predicted MW of 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Human IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Human IL-12, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
The purity of Human IL-12 is greater than 95% as determined by SEC-HPLC.
Human IL-12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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