- Overview
- Specifications
- Shipping, Reconstitution & Storage
- General View of Documents
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Overview
General Description
Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Cys24-Glu540.
Biotinylated
Specifications
Target
IL-12 R beta 1/CD212
Synonyms
IL-12R-beta-1 ; IL-12RB1 ; IL12R ; IL12RB ; CD212 ; IL-12R subunit beta-1 ; IL-12R-BETA1 ; MGC34454
Accession
Species
Human
Exact Sequence
Cys24-Glu540
Molecular Weight
The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity
The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
The purity of Human IL-12 R beta 1 is greater than 95% as determined by SEC-HPLC.
Human IL-12 R beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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