- Overview
- Specifications
- Shipping, Reconstitution & Storage
- General View of Documents
- Declaration
- Quick Inquiry
Overview
General Description
Recombinant Human NKG2C&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Glu98-Leu231(NKG2C)&Ser34-Ile179(CD94).
Biotinylated
Specifications
Target
NKG2C&CD94
Synonyms
NKG2C&CD94
Accession
P26717(NKG2C)&Q13241
Species
Human
Exact Sequence
Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)
Molecular Weight
The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.
Activity
The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
N-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).
The purity of Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC.
Human NKG2C&CD94 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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