Human NY-ESO-1 (HLA-A*02:01) Tetramer Pro­tein, Acces­sion : A0A140T913

• Overview
• Spec­i­fi­ca­tions
• Ship­ping, Recon­sti­tu­tion & Storage
• Gen­er­al View of Documents
• Dec­la­ra­tion
• Quick Inquiry

Overview

Gen­er­al Description

Recom­bi­nant Human NY-ESO-1 (HLA-A*02:01) Tetramer Pro­tein is expressed from E.coli with His tag and Avi tag at the C-terminal,tetramer is assem­bled by biotiny­lat­ed monomer and strep​ta​vidin​.It con­tains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

NY-ESO-1 (HLA-A*02:01) Tetramer

Syn­onyms

Acces­sion

A0A140T913

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 258 kDa. The pro­tein migrates to 160-250 kDa (HLA-A*02:01&strep­ta­vidin tetramer) and 11.9 kDa (B2M) based on Tris-Bis PAGE result under Non reduc­ing (N) condition.

Activ­i­ty

Immo­bi­lized Human NY-ESO-1 (HLA-A*02:01) Tetramer, His Tag at 5μg/​ml (100μl/​well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*02:01) Anti­body, hFc Tag with the EC50 of 7.0ng/ml deter­mined by ELISA. See test­ing image for detail.

Prod­uct Tag

C-His-Avi

Express Sys­tem

E.coli

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per μg by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Liq­uid

Ship­ping Conditions

Shipped with blue ice.

For­mu­la­tion

Sup­plied as 0.22μm fil­tered solu­tion in 20mM Tris, 150mM NaCl (pH 8.0). Please dilute to the desired con­cen­tra­tion accord­ing to the con­cen­tra­tion of the solu­tion shown on the prod­uct label.

Recon­sti­tu­tion

Sta­bil­i­ty & Storage

Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the pro­tein into small­er quan­ti­ties for opti­mal stor­age. Please do not repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Immo­bi­lized Human NY-ESO-1 (HLA-A*02:01) Tetramer, His Tag at 5μg/​ml (100μl/​well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*02:01) Anti­body, hFc Tag with the EC50 of 7.0ng/ml deter­mined by ELISA.

The puri­ty of Human NY-ESO-1 (HLA-A*02:01) Tetramer is greater than 95% as deter­mined by SEC-HPLC.

Human NY-ESO-1 (HLA-A*02:01) Tetramer on Tris-Bis PAGE under Non reduc­ing (N) con­di­tion. The puri­ty is greater than 95%.

Dec­la­ra­tion

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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