sds page 105 03 3 340x400 - Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade (rHuLR3IGF-1,MediaGrade) CAS 105-03-1816

Offi­cial Full Name

Recom­bi­nant Human LR3 Insulin-like Growth fac­­tor-1, Media Grade (rHuLR3IGF-1,MediaGrade)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

105-03-1816

Syn­onyms

LR3 IGF-I

Squence

region 105 03 - Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade (rHuLR3IGF-1,MediaGrade) CAS 105-03-1816

Amino Acid Sequence

MFPAM­PLSSL FVNG­PRTL­CG AELV­DALQFV CGDRGFYFNK PTGYGSSS­RR APQT­GIVDEC CFRSCDL­R­RL EMY­CAPLK­PA KSA

Syn­onyms

LR3 IGF-I

Acces­sion

Gene ID

Sum­ma­ry

IGF-1 belonged to the insulin gene fam­i­ly, is a mito­genic polypep­tide growth fac­tor that stim­u­late the pro­lif­er­a­tion and sur­vival of var­i­ous cell types includ­ing mus­cle, bone, and car­ti­lage tis­sue in vit­ro. It is pro­duced pri­mar­i­ly by the liv­er as an endocrine hor­mone as well as in tar­get tis­sues in a paracrine/​autocrine fash­ion. The pro­duc­tion of IGF-1 is stim­u­lat­ed by growth hor­mone (GH) and can be retard­ed by under­nu­tri­tion, growth hor­mone insen­si­tiv­i­ty, lack of growth hor­mone recep­tors, or fail­ures of the down­stream sig­nal­ing path­way post GH recep­tor includ­ing SHP2 and STAT5B. The LR3IGF-1 is a long-term ana­log of human IGF-1, specif­i­cal­ly designed and man­u­fac­tured for mam­malian cell cul­ture to sup­port large-scale man­u­fac­tur­ing of recom­bi­nant bio­phar­ma­ceu­ti­cals.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 9.1 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 83 amino acids.

Bio­log­i­cal Activity

Assay 1 : Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. Mea­sured in a serum-free cell pro­lif­er­a­tion assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 6.7 × 105 U/​mg. Assay 2 : Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by the stim­u­la­tion of pro­tein syn­the­sis using rat L6 myoblasts is less than 10 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 1.0 × 105 U/​mg.

Appear­ance

Ster­ile Fil­tered White lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in 20 mM PB, pH 7.2.

Endo­tox­in

Less than 0.01 EU/​ug of rHuLR3 IGF-1 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.-Refer to lot spe­cif­ic COA for the Use by Date when stored at 2 to 8 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

1. Con­lon MA, Tomas FM, Owens PC, et al. 1995. J Endocrinol, 146 : 247-53.2. Dunais­ki V, Dun­shea FR, Wal­ton PE, et al. 1997. J Endocrinol, 155 : 559-65.3. Gar­naut SM, Howarth GS, Read LC. 2002. Growth Fac­tors, 20 : 17-25.4. Xi G, Kaman­­ga-Sol­­lo E, Pam­pusch MS, et al. 2004. J Cell Phys­i­ol, 200 : 387-94.5. Had­sell DL, Par­low AF, Tor­res D, et al. 2008. J Endocrinol, 198 : 61-70.

Puri­ty

> 98 % by SDS-PAGE analyses.>90 % by RP-HPLC analy­ses.

SDS-PAGE

sds page 105 03 2 - Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade (rHuLR3IGF-1,MediaGrade) CAS 105-03-1816

Pack Size

1mg/​5mg/​20mg

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

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Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human LR3 Insulin-like Growth fac­­tor-1, Media Grade (rHuLR3IGF-1,MediaGrade) on ChemWhat

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