Identification
CAS Number
9001-60-9
EC
1.1.1.27
Name
LLCD, L-Lactic dehydrogenase
Source
Microorganism
Molecular weight
38 kDa (SDS-PAGE)
EINECS
232-617-8
Properties
Appearance
White amorphous powder
Isoelectric point
6.2
Michaelis constant
1.3×10 M(Pyruvate)
-6 4.0×10 M(NADH)
Safety Data
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our LLCD CAS 9001-60-9
Specific activity
≥ 300U/mg protein
Unit definition
One unit will convert one micromole of pyruvate to L-lactate per min at pH 7.4 at 25℃.
Optimum pH
6.5
Optimum temperature
45℃
pH Stability
4.5~10.0 (37℃,1hr)
Thermal stability
< 50℃ (pH 7.4, 15min)
Inhibitors
Co ,Cu ,Fe ,Ni ,Zn ,NEM,SDS,Proclin
Effect of Various Chemicals on L-LDH
The enzyme dissolved in 50mM Tris-HCl buffer, pH 7.5 (20U/ml) was incubated with each chemical at 37℃ for 2hr
| Chemical | Concn.（mM） | Residual activity |
| None | 2.0 | 100% |
| CaCl2 | 2.0 | 101% |
| CoCl2 | 2.0 | 50% |
| CuSO4 | 2.0 | 0% |
| FeCl3 | 2.0 | 27% |
| MgSO4 | 2.0 | 94% |
| MnSO4 | 2.0 | 96% |
| NiCl2 | 2.0 | 78% |
| ZnSO4 | 2.0 | 0% |
| K4Fe(CN)6 | 2.0 | 98% |
| BME | 2.0 | 98% |
| NME | 2.0 | 79% |
| EDTA | 5.0 | 102% |
| NaN3 | 20.0 | 101% |
| Proclin | 0.045% | 58% |
| Na-cholate | 0.10% | 109% |
| SDS | 0.05% | 0% |
| Triton X-100 | 0.10% | 113% |
| Tween 20 | 0.10% | 115% |
Shelf Life
2 years
Storage
Store at -20℃ for long time.
Known Application
For biochemical research, determination of pyruvate, diagnosis of leukemia and myocardial infarction.
Links
This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).
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