compound no - LLCD CAS 9001-60-9

Iden­ti­fi­ca­tion

CAS Number

9001-60-9

EC

1.1.1.27

Name

LLCD, L-Lac­tic dehydrogenase

Source

Microor­gan­ism

Mol­e­c­u­lar weight

38 kDa (SDS-PAGE)

EINECS

232-617-8

Prop­er­ties

Appear­ance

White amor­phous powder

Iso­elec­tric point

6.2

Michaelis con­stant

1.3×10 M(Pyruvate)
-6 4.0×10 M(NADH)

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our LLCD CAS 9001-60-9

Spe­cif­ic activity

300U/​mg protein

Unit def­i­n­i­tion

One unit will con­vert one micro­mole of pyru­vate to L-lac­­tate per min at pH 7.4 at 25℃.

Opti­mum pH

6.5

Opti­mum temperature

45℃

pH Sta­bil­i­ty

4.5~10.0 (37℃,1hr)

Ther­mal stability

< 50℃ (pH 7.4, 15min)

Inhibitors

Co ,Cu ,Fe ,Ni ,Zn ,NEM,SDS,Pro­clin

Effect of Var­i­ous Chem­i­cals on L-LDH

The enzyme dis­solved in 50mM Tris-HCl buffer, pH 7.5 (20U/​ml) was incu­bat­ed with each chem­i­cal at 37℃ for 2hr

Chem­i­calConcn.(mM)Resid­ual activity
None2.0100%
CaCl22.0101%
CoCl22.050%
CuSO42.00%
FeCl32.027%
MgSO42.094%
MnSO42.096%
NiCl22.078%
ZnSO42.00%
K4Fe(CN)62.098%
BME2.098%
NME2.079%
EDTA5.0102%
NaN320.0101%
Pro­clin0.045%58%
Na-cholate0.10%109%
SDS0.05%0%
Tri­ton X-1000.10%113%
Tween 200.10%115%
LLCD CAS 9001 60 9 - LLCD CAS 9001-60-9

Shelf Life

2 years

Stor­age

Store at -20℃ for long time.

Known Appli­ca­tion

For bio­chem­i­cal research, deter­mi­na­tion of pyru­vate, diag­no­sis of leukemia and myocar­dial infarction.

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd (http://​www​.ulcho​.com/).

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