Identification
CAS Number
66676-43-5
EC Number
3.4.21.19
Name
Recombinant V8 protease
Synonyms
STAPHYLOCOCCAL SERINE PROTEASE PROTEASE V8 ;
STAPHYLOCOCCAL SERINE PROTEINASE ;
PROTEASE V8 ;
PROTEASE, S AUREUS ;
PROTEASE TYPE XVII-B ;
V8 PROTEASE ;
EC 3.4.21.19 ;
ENDOPROTEINASE GLU-C
EINECS
620-751-3
Properties
Appearance
White or off white, or yellowish powder
Safety Data
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our Recombinant V8 protease EC 3.4.21.19 CAS 66676-43-5
Purity(SDS-PAGE)
Single major band
Specific activity
≥ 5.0 AU /mg pro
Molecular Weight(SDS-PAGE)
24.0±2.4 kDa
Unit definition
One unit is defined as the amount of enzyme required to catalytic substrate Z-Phe-Leu-Glu-4-nitranilide produced 1μmol of 4-nitroaniline per minute at 25°C and pH 7.8
Description
Recombinant V8 protease is the serine protease family and can specifically hydrolyzing glutamic acid or aspartic acid residues carboxy side peptide bonds. The Glu carboxy terminal peptide bond can be identified and cleaved in CH4COONH4 buffer (pH 7.8) and NH4HCO3 (pH 4.0 ).Identify and cleave the Glu or Asp carboxy terminal peptide bond in phosphate buffer (pH 7.8),and the hydrolysis rate of Glu was higher than that of Glu Asp. Recombinant V8 protease has activity between pH 4.0-10.0, and the optimum pH is 8.0-8.5. It can be used alone or in combination with other proteases when used in protein digestion, peptide profiling and peptide mass fingerprinting analysis. The inhibitors of V8 protease are diisopropylfluorophosphate (DFP), α2-macroglobulin and Nα-P-tosyl-L-lysine chloromethyl ketone (TLCK).
Source
Recombinant V8 protease is a genetically engineered protein expressed in E.coli
Protein Sequence
The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin
Recommended usage
Note that before using : (1) Divide the material in according to usage quantity to avoid pollution or self-cutting. (2) Dissolve target protein.
The target protein is dissolved by cleavage buffer, for example 25-50 mM NH4HCO3 pH 7.8, if the solubility of target protein is not good, the target protein will be denatured by adding urea, SDS, DTT or heating. The effects of urea and SDS on V8 protease are shown in.(3) Digest : The recommended ratio of V8 protease and target protein is 1:20-1:100 (W/W).At 25 °C or 37 °C digest the protein 2-18h.
Stability of storage
Recombinant V8 protease should be stored under 2-8°C in sealed container.It is stable within 6 months after dissolved with1mM HCl and 50mM HAC at -20°C or below. It is stable within 2 months after dissolved with 1mM HCl and 50mM HAC at 4°C.85% activity above can be kept after dissolved with 1mM HCl and 50mM HAC at 25°C or 37°C 12h. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport
The product is stable by blue ice insulation transport.
Advantage
1.Sequence grade recombinant protein : high specificity,good stability.
2.Animal origin free : Recombinant V8 Protease is no exogenous virus contamination,and any animal origin material is not used in the production process.
3.Stable quality : Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
4.High purity : Higher specific activity.Host protein residues is less than the limits of biological products.
