Recom­bi­nant Apro­tinin CAS 9087-70-1

• Iden­ti­fi­ca­tion
• Prop­er­ties
• Safe­ty Data
• Spec­i­fi­ca­tions & Oth­er Information
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Iden­ti­fi­ca­tion

CAS Number

9087-70-1

Name

Recom­bi­nant Aprotinin

Syn­onyms

[9087-70-1]
232-994-9 [EINECS]
9004-04-0 [RN]
9087-70-1 [RN]
Antikrein ; Antilysin
Apro­tinin
Apro­tinin from bovine lung
MFCD00130541
YN 5080000
YN5080000

InChI

InChI=1S/C284H432N84O79S7/c1-21-144(9)222-271(439)337-174(68-46-105-309-282(300)301)239(407)340-187(120-160-77-85-164(374)86-78-160)251(419)341-185(116-156-55-29-24-30-56-156)250(418)342-188(121-161-79-87-165(375)88-80-161)252(420)346-191(123-208(291)378)246(414)322-149(14)230(398)326-168(62-35-39-98-285)234(402)319-146(11)227(395)314-132-215(385)324-181(113-141(3)4)247(415)354-199-137-452-453-138-200-263(431)336-179(97-112-448-20)242(410)331-176(70-48-107-311-284(304)305)244(412)363-226(154(19)372)274(442)358-197(233(401)316-129-212(382)312-130-213(383)318-151(16)278(446)447)135-449-451-139-201(355-253(421)186(117-157-57-31-25-32-58-157)344-256(424)195(127-220(393)394)350-267(435)204-72-50-109-366(204)275(443)167(289)61-43-102-306-279(294)295)265(433)339-182(114-142(5)6)248(416)338-180(93-96-218(389)390)276(444)368-111-52-74-206(368)277(445)367-110-51-73-205(367)268(436)349-189(122-162-81-89-166(376)90-82-162)259(427)362-224(152(17)370)269(437)317-133-216(386)365-108-49-71-203(365)266(434)357-202(264(432)333-169(63-36-40-99-286)235(403)320-148(13)229(397)328-175(69-47-106-310-283(302)303)243(411)360-223(145(10)22-2)272(440)361-222)140-454-450-136-198(325-214(384)131-313-211(381)128-315-232(400)183(119-159-75-83-163(373)84-76-159)351-270(438)221(143(7)8)359-258(426)190(118-158-59-33-26-34-60-158)352-273(441)225(153(18)371)364-245(413)177(335-262(199)430)91-94-207(290)377)261(429)334-172(66-44-103-307-280(296)297)236(404)321-147(12)228(396)327-170(64-37-41-100-287)237(405)330-173(67-45-104-308-281(298)299)238(406)345-192(124-209(292)379)255(423)347-193(125-210(293)380)254(422)343-184(115-155-53-27-23-28-54-155)249(417)332-171(65-38-42-101-288)240(408)353-196(134-369)260(428)323-150(15)231(399)329-178(92-95-217(387)388)241(409)348-194(126-219(391)392)257(425)356-200/h23-34,53-60,75-90,141-154,167-206,221-226,369-376H,21-22,35-52,61-74,91-140,285-289H2,1-20H3,(H2,290,377)(H2,291,378)(H2,292,379)(H2,293,380)(H,312,382)(H,313,381)(H,314,395)(H,315,400)(H,316,401)(H,317,437)(H,318,383)(H,319,402)(H,320,403)(H,321,404)(H,322,414)(H,323,428)(H,324,385)(H,325,384)(H,326,398)(H,327,396)(H,328,397)(H,329,399)(H,330,405)(H,331,410)(H,332,417)(H,333,432)(H,334,429)(H,335,430)(H,336,431)(H,337,439)(H,338,416)(H,339,433)(H,340,407)(H,341,419)(H,342,418)(H,343,422)(H,344,424)(H,345,406)(H,346,420)(H,347,423)(H,348,409)(H,349,436)(H,350,435)(H,351,438)(H,352,441)(H,353,408)(H,354,415)(H,355,421)(H,356,425)(H,357,434)(H,358,442)(H,359,426)(H,360,411)(H,361,440)(H,362,427)(H,363,412)(H,364,413)(H,387,388)(H,389,390)(H,391,392)(H,393,394)(H,446,447)(H4,294,295,306)(H4,296,297,307)(H4,298,299,308)(H4,300,301,309)(H4,302,303,310)(H4,304,305,311)

InChI Key

ZPN­FWUPYT­F­PO­JU-UHF­F­­FAOYSA-N

SMILES

CCC(C)C1C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NCC(=O)NC(C(=O)NC2CSSCC3C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(CSS­CC(C(=O)NC(C(=O)NC(C(=O)N4CCCC4C(=O)N5CCCC5C(=O)NC(C(=O)NC(C(=O)NCC(=O)N6CCCC6C(=O)NC(CSS­CC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C(=O)N3)CC(=O)O)CCC(=O)O)C)CO)CCC­CN)CC7=CC=CC=C7)CC(=O)N)CC(=O)N)CCC­NC(=N)N)CCC­CN)C)CCC­NC(=N)N)NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC2=O)CCC(=O)N)C(C)O)CC8=CC=CC=C8)C(C)C)CC9=CC=C(C=C9)O)C(=O)NC(C(=O)NC(C(=O)NC(C(=O)N1)CCC­NC(=N)N)C)CCC­CN)C(C)O)CC1=CC=C(C=C1)O)CCC(=O)O)CC(C)C)NC(=O)C(CC1=CC=CC=C1)NC(=O)C(CC(=O)O)NC(=O)C1CCCN1C(=O)C(CCC­NC(=N)N)N)C(=O)NCC(=O)NCC(=O)NC(C)C(=O)O)C(C)O)CCC­NC(=N)N)CCSC)CC(C)C)C)CCC­CN)C)CC(=O)N)CC1=CC=C(C=C1)O)CC1=CC=CC=C1)CC1=CC=C(C=C1)O)CCC­NC(=N)N)C(C)CC

Mol­e­c­u­lar Formula

C₂₈₄H₄₃₂N₈₄O₇₉S₇

Mol­e­c­u­lar Weight

6511.44

EINECS

232-994-9

MDL Number

MFCD00130541

Prop­er­ties

Appear­ance

White or off white, or yel­low­ish powder

Safe­ty Data

Per­son­al Pro­tec­tive Equipment

Eye­shields, Gloves, type N95 (US)

RIDADR 

NONH for all modes of transport

WGK Germany

1

RTECS

YN5080000

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Recom­bi­nant Apro­tinin CAS 9087-70-1

Sol­u­bil­i­ty (5mg/​ml in 0.9%NaCl)

Clear to very slight hazy

Pro­tein Con­tent [E1% A280 nm = 8.4]

≥60%

Puri­ty(HPLC)

≥95%

Spe­cif­ic activ­i­ty(EPU /​mg protein)

≤ 0.8(3 EPU/​ml in water)

Absorbance (277nm)

≤0.8 (3EPU/​ml in water)

Unit def­i­n­i­tion

One trypsin inhibitor unit (EPU) will decrease the activ­i­ty of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-ben­­zoyl-L-argini­neethyl ether(BAEE) per sec at pH 7.6 at 25 °C.

Descrip­tion

Apro­tinin is a com­pet­i­tive ser­ine pro­tease inhibitor that inhibits trypsin, chy­motrypsin, kallikrein and plas­min. Apro­tinin forms sta­ble com­plex­es with and blocks the active sites of enzymes. Bind­ing is reversible with most apro­­tinin-pro­tease com­plex­es and dis­so­ci­at­ing at pH <3.0. Effec­tive con­cen­tra­tion is equimo­lar with pro­tease. Yax­in­Bio recom­bi­nant apro­tinin is a genet­i­cal­ly engi­neered pro­tein expressed in E.coli and purified by multi-column.The amino acid sequence of recom­bi­nant trypsin is iden­ti­cal to bovine lung derived aprotinin,with equiv­a­lent prop­er­ties com­pared to native apro­tinin and can replace native trypsin for using in a vari­ety of biotech­no­log­i­cal processes.

Source

Recom­bi­nant apro­tinin is a genet­i­cal­ly engi­neered pro­tein expressed in E.coli

Pro­tein Sequence

The amino acid sequence of recom­bi­nant apro­tinin is iden­ti­cal to bovine lung derived aprotinin.

Rec­om­mend­ed usage

Effec­tive con­cen­tra­tion is equimo­lar with pro­tease. The rec­om­mend bind­ing is pH＞6.0 and it will dis­so­ci­ate at pH <3.0. Dis­solve pow­der in 0.9%NaCl,then the liq­uid should be stored at -20℃ or below.

Sta­bil­i­ty of storage

Recom­bi­nant tapro­tinin lyophilized should be stored under 2-8℃ in sealed con­tain­er. It is sta­ble with­in 24 months.

Sta­bil­i­ty of transport

The prod­uct is sta­ble by blue ice insu­la­tion transport.

Advan­tage

1. Ani­mal ori­gin free : recom­bi­nant trypsin is no exoge­nous virus contamination,and any ani­mal ori­gin mate­r­i­al is not used in the pro­duc­tion process.
2. Sta­ble qual­i­ty : Mass pro­duc­tion can ensure sta­ble and con­tin­u­ous batch pro​duc​tion​.It is no dif­fer­ence between the batch and the prod­uct qual­i­ty is stable.
3. High puri­ty : High­er spe­cif­ic activ​i​ty​.Host pro­tein residues is less than the lim­its of bio­log­i­cal products.
4. Lyophilized pow­der : The prod­uct is lyophilized pow­der and is easy to store and transport.

Known Appli­ca­tion

Apro­tinin is a com­pet­i­tive ser­ine pro­tease inhibitor that inhibits trypsin, chy­motrypsin, kallikrein and plas­min. Apro­tinin forms sta­ble com­plex­es with and blocks the active sites of enzymes. Recom­bi­nant apro­tinin is with equiv­a­lent prop­er­ties com­pared to native apro­tinin and can replace native trypsin for using in a vari­ety of biotech­no­log­i­cal processes,such as inhi­bi­tion of ser­ine pro­tease activ­i­ty in recom­bi­nant pro­tein production,cell cul­ture and so on.

Links

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd(https://​www​.wat​son​-bio​.com/), and here is the cor­re­spond­ing linkhttps://​www​.wat​son​-bio​.com/​r​e​c​o​m​b​i​n​a​n​t​-​a​p​r​o​t​i​n​i​n​-​c​a​s​-​9​0​8​7​-​7​0​-1/

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