Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17) CAS 103-10-1816 Watson International Limited

Official Full Name

Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17)

CAS Number (Or Watson Number for Non-CAS Products)

103-10-1816

Synonyms

TNFRSF17, BCM, CD269

Squence

region 103 10 1 - Recombinant Human B-Cell Maturation Antigen/TNFRSF17 (rHuBCMA/TNFRSF17) CAS 103-10-1816

Amino Acid Sequence

AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA

Synonyms

TNFRSF17, BCM, CD269

Accession

Q02223

Gene ID

608

Summary

Source

Escherichia coli.

Molecular Weight

Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 100 ng/ml human APRIL.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.

Endotoxin

Less than 1 EU/ug of rHuBCMA as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

References

1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44 : 69-81.2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346 : 1158-62.3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278 : 33127-33.4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22 : 1147-54.