Recom­bi­nant Human Beta­cel­lulin (rHu­Be­ta­cel­lulin) CAS 107-12-1816

Offi­cial Full Name

Recom­bi­nant Human Beta­cel­lulin (rHu­Be­ta­cel­lulin)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

107-12-1816

Syn­onyms

Squence

Amino Acid Sequence

DGN­STR­SPET NGLL­CGDPEE NCAATT­TQSK RKGHF­S­R­CPK QYKHY­CIK­GR CRFV­VAE­QTP SCVCD­E­GYIG ARCERVDL­FY

Syn­onyms

Acces­sion

P35070

Gene ID

685

Sum­ma­ry

Beta­cel­lulin (BTC) is a mem­ber of the EGF fam­i­ly of cytokines that also includes EGF, TGF-a, Amphireg­ulin, HB-EGF, Epireg­ulin, Tomoreg­ulin and the Neureg­ulins. It is expressed in most tis­sues includ­ing kid­ney, uterus, liv­er and pan­creas. BTC also presents in body flu­ids, includ­ing serum, milk, and colostrum. At the amino acid sequence lev­el, human mature BTC pro­tein exhibits 80 % iden­ti­ty with mouse BTC pro­tein. The pro­tein binds to EGFR, ERBB4 and oth­er EGF recep­tor fam­i­ly mem­bers and acts as a potent mito­gen for reti­nal pig­ment epithe­lial cells and vas­cu­lar smooth mus­cle cells.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Recom­bi­nant human Beta­cel­lulin is a 9.0 kDa monomer­ic pro­tein, con­tain­ing 80 amino residues, which com­pris­es the mature EGF homol­o­gous por­tion of the Beta­cel­lulin pro­tein.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by a cell pro­lif­er­a­tion assay using murine Balb/​c 3T3 cells is less than 0.05 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 2.0 × 107 IU/​mg.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in PBS, pH 7.4.

Endo­tox­in

Less than 1 EU/​ug of rHuBTC as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 98 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Beta­cel­lulin (rHu­Be­ta­cel­lulin) on ChemWhat

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