Identification
CAS Number
91932-65-9
Name
Recombinant Protein A
Properties
Appearance
White or off white powder
Safety Data
WGK Germany
1
Specifications and Other Information of Our Recombinant Protein A CAS 91932-65-9
Purity (HPLC)
≥95%
Solubility
Clear liquid
Bioburden
≤1 cfu/mg
UV Spectrum
E275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
SDS-PAGE
Single major band
SDS-PAGE (MW)
25000±1000 Daltons
Description
Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.
Source
Protein A mutant of Staphylococcus aureus , expressed in E. coli
Stability of storage
Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use. After dissolved with pure water, it should be stored under -20℃.
Stability of transport
The product is stable by blue ice insulation transport.
Advantage
1.Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.
2.SPA-25 pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.
3.The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.
4.Stable quality : Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
5.High purity : Higher specific activity.Host protein residues is less than the limits of biological products.
Known Application
The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.
