Structure of Recombinant Proteinase K EC 3.4.21.14 CAS 39450 01 6 - Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6

Iden­ti­fi­ca­tion

CAS Number

39450-01-6

EC Number

3.4.21.14

Name

Recom­bi­nant Pro­teinase K

Syn­onyms

1H-Ben­z­im­i­­da­­zole-5-car­box­am­ide, 1-(2-methoxyethyl)-N-[1-methyl-2-(1-methylethyl)-1H-benzimidazol-5-yl]- [ACD/​Index Name]
N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazol-5-carboxamid [Ger­man] [ACD/IUPAC Name]
N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazole-5-carboxamide [ACD/IUPAC Name]
N-(2-Isopropyl-1-méthyl-1H-benzimidazol-5-yl)-1-(2-méthoxyéthyl)-1H-benzimidazole-5-carboxamide [French] [ACD/IUPAC Name]
1-(2-methoxyethyl)-N-(1-methyl-2-propan-2-ylbenzimidazol-5-yl)benzimidazole-5-carboxamide
1214393-36-8 [RN]
39450-01-6 [RN]
pro­teinase k, lyophilized pow­der, biotechgrade

SMILES

CC(C1C(=O)N2C1(C(C(=C2C(=O)OCC3=CC=C(C=C3)[N+](=O)[O-])OC4=CC=CC=C4)(C)OC5=CC=CC=C5)OP(=O)(O)O)O

InChI

InChI=1S/C29H27N2O12P/c1-18(32)23-26(33)30-24(27(34)40-17-19-13-15-20(16-14-19)31(35)36)25(41-21-9-5-3-6-10-21)28(2,29(23,30)43-44(37,38)39)42-22-11-7-4-8-12-22/h3-16,18,23,32H,17H2,1-2H3,(H2,37,38,39)/t18-,23-,28-,29+/m1/s1

InChI Key

OUPAKHMWU­JJX­OM-ANOMEZDXSA-N

Mol­e­c­u­lar Formula

C22H25N5O2

Mol­e­c­u­lar Weight

391.466

EINECS

254-457-8

MDL Number

MFCD00132129

Prop­er­ties

Appear­ance

Lyophilized

Safe­ty Data

Sym­bol

exclamation mark jpg - Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6health hazard jpg - Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6GHS07,GHS08

Sig­nal Word

Dan­ger

Haz­ard statements

H315H319H334H335

Pre­cau­tion­ary Statements

P302 + P352P305 + P351P338

Tar­get organs

Res­pi­ra­to­ry system

Per­son­al Pro­tec­tive Equipment

dust mask type N95 (US), Eye­shields, Faceshields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

1

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Recom­bi­nant Pro­teinase K EC 3.4.21.14 CAS 39450-01-6

Puri­ty

≥95%

Spe­cif­ic activity

≥30 units/​mg

Mol­e­c­u­lar Weight(SDS-PAGE)

29.3kD

Unit def­i­n­i­tion

One unit is defined as the amount of enzyme that will hydrolyze urea-dena­­tured hemo­glo­bin to pro­duce col­or equiv­a­lent to 1.0 mol tyro­sine per min at 37°C, pH 7.5(color by Folin-Cio­­cal­­teu reagent).

Descrip­tion

A ser­ine pro­tease that dis­plays the abil­i­ty to digest native pro­teins, there­by inac­ti­vat­ing enzymes such as DNase and RNase with­out recourse to a denat­u­ra­tion process. It retains its activ­i­ty in pres­ence of SDS and urea. It is inac­ti­vat­ed by diiso­propyl flu­o­rophos­phates (DFP) and phenyl methane sul­fonyl flu­o­ride (PMSF).

Rec­om­mend­ed usage

Pre­pare recom­bi­nant pro­teinase K with 20mM Tris-HCl (pH 7.4~8.0),1mM CaCl,50% glycerol.The opti­mum pH is 8.0.

Stor­age

Store lyophilized at -20°C. Aliquot the prod­uct after recon­sti­tu­tion to avoid repeat­ed freez­ing thaw­ing cycles.

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd(https://​www​.wat​son​-bio​.com/), and here is the cor­re­spond­ing linkhttps://​www​.wat​son​-bio​.com/​r​e​c​o​m​b​i​n​a​n​t​-​p​r​o​t​e​i​n​a​s​e​-​k​-​e​c​-​3​-​4​-​2​1​-​1​4​-​c​a​s​-​3​9​4​5​0​-​0​1​-6/

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