Structure of Recombinant Proteinase K EC 3.4.21.14 CAS 39450 01 6 - Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6

Identification

CAS Number

39450-01-6

EC Number

3.4.21.14

Name

Recombinant Proteinase K

Synonyms

1H-Benzimidazole-5-carboxamide, 1-(2-methoxyethyl)-N-[1-methyl-2-(1-methylethyl)-1H-benzimidazol-5-yl]- [ACD/Index Name]
N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazol-5-carboxamid [German] [ACD/IUPAC Name]
N-(2-Isopropyl-1-methyl-1H-benzimidazol-5-yl)-1-(2-methoxyethyl)-1H-benzimidazole-5-carboxamide [ACD/IUPAC Name]
N-(2-Isopropyl-1-méthyl-1H-benzimidazol-5-yl)-1-(2-méthoxyéthyl)-1H-benzimidazole-5-carboxamide [French] [ACD/IUPAC Name]
1-(2-methoxyethyl)-N-(1-methyl-2-propan-2-ylbenzimidazol-5-yl)benzimidazole-5-carboxamide
1214393-36-8 [RN]
39450-01-6 [RN]
proteinase k, lyophilized powder, biotechgrade

SMILES

CC(C1C(=O)N2C1(C(C(=C2C(=O)OCC3=CC=C(C=C3)[N+](=O)[O-])OC4=CC=CC=C4)(C)OC5=CC=CC=C5)OP(=O)(O)O)O

InChI

InChI=1S/C29H27N2O12P/c1-18(32)23-26(33)30-24(27(34)40-17-19-13-15-20(16-14-19)31(35)36)25(41-21-9-5-3-6-10-21)28(2,29(23,30)43-44(37,38)39)42-22-11-7-4-8-12-22/h3-16,18,23,32H,17H2,1-2H3,(H2,37,38,39)/t18-,23-,28-,29+/m1/s1

InChI Key

OUPAKHMWUJJXOM-ANOMEZDXSA-N

Molecular Formula

C22H25N5O2

Molecular Weight

391.466

EINECS

254-457-8

MDL Number

MFCD00132129

Properties

Appearance

Lyophilized

Safety Data

Symbol

exclamation mark jpg - Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6health hazard jpg - Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6GHS07,GHS08

Signal Word

Danger

Hazard statements

H315 – H319 – H334 – H335

Precautionary Statements

P302 + P352 – P305 + P351 + P338

Target organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

1

Specifications and Other Information of Our Recombinant Proteinase K EC 3.4.21.14 CAS 39450-01-6

Purity

≥95%

Specific activity

≥30 units/mg

Molecular Weight(SDS-PAGE)

29.3kD

Unit definition

One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).

Description

A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).

Recommended usage

Prepare recombinant proteinase K with 20mM Tris-HCl (pH 7.4~8.0),1mM CaCl,50% glycerol.The optimum pH is 8.0.

Storage

Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.

This product is developed by our R&D company Watson Bio Ltd(https://www.watson-bio.com/), and here is the corresponding linkhttps://www.watson-bio.com/recombinant-proteinase-k-ec-3-4-21-14-cas-39450-01-6/

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