Structure of Recombinant Protein G CAS UENA 0187 - Recombinant Protein G CAS UENA-0187

Iden­ti­fi­ca­tion

CAS Number

UENA-0187

Name

Recom­bi­nant Pro­tein G

Prop­er­ties

Appear­ance

White, yel­low­ish pow­der or floc

Safe­ty Data

WGK Germany

1

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Recom­bi­nant Pro­tein G CAS UENA-0187

Puri­ty

≥95%

A280nm (1 mg / mL)

1.350

Iso­elec­tric point

4.79

Mol­e­c­u­lar weight

28 kDa

Descrip­tion

Strep­to­coc­cus Pro­tein G (SPG for short), is a kind of pro­tein in the cell wall of Strep­to­coc­cus G and Strep­to­coc­cus C, which can specif­i­cal­ly bind to the Fc seg­ment of an immunoglob­u­lin IgG mol­e­cule with­out affect­ing the abil­i­ty of its Fab seg­ment to bind to the anti­gen mol­e­cule. It can not only bind to all sub­class­es of human, mouse, and rat IgG, but also to guinea pig, rab­bit, goat, cow, sheep, and horse IgG, which is sig­nif­i­cant­ly bet­ter than Pro­tein A (Staphy­lo­coc­cus Pro­tein A, SPA for short) that can only bind to human and mouse IgG.
In addi­tion to the IgG bind­ing domain, nat­ur­al SPGs also have albu­min and cell sur­face bind­ing domains. Recom­bi­nant SPG removes albu­min and cell sur­face bind­ing domains to reduce non-spe­­cif­ic bind­ing. It has greater affin­i­ty than nat­ur­al SPG and SPA.

Source

Pro­duced by genet­ic engi­neer­ing, expressed by E.Coli

Sta­bil­i­ty of storage

The lyophilized pow­der of Recom­bi­nant Strep­to­coc­cus Pro­tein G was stored at -20℃, and was sta­ble for 60 months ; after dis­solv­ing, put it on an ice bath and use it as soon as pos­si­ble. If you need to use it mul­ti­ple times, please split and store it at -65℃ before using to avoid repeat­ed freeze-thaw affect­ing pro­tein activity ;

Sta­bil­i­ty of transport

Blue ice insu­la­tion trans­porta­tion, sta­ble activity.

Known Appli­ca­tion

SPG has a wide range of uses and can be used to sep­a­rate and puri­fy or remove IgG from serum, the purifi­ca­tion of mon­o­clon­al anti­bod­ies and the sep­a­ra­tion of anti­­gen-anti­­body com­plex­es. After bind­ing SPG to agarose gel, affin­i­ty fillers can be pre­pared for anti­body purifi­ca­tion, and dif­fer­ent sub­types of IgG can be iso­lat­ed and puri­fied from serum, ascites, and cell cul­ture supernatant.

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd(https://​www​.wat​son​-bio​.com/), and here is the cor­re­spond­ing linkhttps://​www​.wat​son​-bio​.com/​r​e​c​o​m​b​i​n​a​n​t​-​p​r​o​t​e​i​n​-​g​-​c​a​s​-​u​e​n​a​-​0​1​87/

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