Identification
CAS Number
UENA-0187
Name
Recombinant Protein G
Properties
Appearance
White, yellowish powder or floc
Safety Data
WGK Germany
1
Specifications and Other Information of Our Recombinant Protein G CAS UENA-0187
Purity
≥95%
A280nm (1 mg / mL)
1.350
Isoelectric point
4.79
Molecular weight
28 kDa
Description
Streptococcus Protein G (SPG for short), is a kind of protein in the cell wall of Streptococcus G and Streptococcus C, which can specifically bind to the Fc segment of an immunoglobulin IgG molecule without affecting the ability of its Fab segment to bind to the antigen molecule. It can not only bind to all subclasses of human, mouse, and rat IgG, but also to guinea pig, rabbit, goat, cow, sheep, and horse IgG, which is significantly better than Protein A (Staphylococcus Protein A, SPA for short) that can only bind to human and mouse IgG.
In addition to the IgG binding domain, natural SPGs also have albumin and cell surface binding domains. Recombinant SPG removes albumin and cell surface binding domains to reduce non-specific binding. It has greater affinity than natural SPG and SPA.
Source
Produced by genetic engineering, expressed by E.Coli
Stability of storage
The lyophilized powder of Recombinant Streptococcus Protein G was stored at -20℃, and was stable for 60 months ; after dissolving, put it on an ice bath and use it as soon as possible. If you need to use it multiple times, please split and store it at -65℃ before using to avoid repeated freeze-thaw affecting protein activity ;
Stability of transport
Blue ice insulation transportation, stable activity.
Known Application
SPG has a wide range of uses and can be used to separate and purify or remove IgG from serum, the purification of monoclonal antibodies and the separation of antigen-antibody complexes. After binding SPG to agarose gel, affinity fillers can be prepared for antibody purification, and different subtypes of IgG can be isolated and purified from serum, ascites, and cell culture supernatant.
