Overview

Gen­er­al Description

Recom­bi­nant Biotiny­lat­ed Mouse IL-5 Pro­tein is expressed from HEK293 with His tag and Avi tag at the N​-ter​mi​nal​.It con­tains Met21-Gly133.

Biotiny­lat­ed

Yes

Spec­i­fi­ca­tions

Tar­get

IL-5

Syn­onyms

Inter­leukin-5 ; IL-5 ; T-cell replac­ing fac­tor ; TRF ; EDF

Acces­sion

P04401

Species

Mouse

Exact Sequence

Met21-Gly133

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 16.0 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 16 kDa and 20-35 kDa based on Tris-Bis PAGE result.

Activ­i­ty

The affin­i­ty con­stant of 0.511 uM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

N-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Liq­uid

Ship­ping Conditions

Shipped with blue ice.

For­mu­la­tion

Sup­plied as 0.22um fil­tered solu­tion in 20mM Tris, 500mM NaCl, 10% Glyc­erol (pH 7.0).

Recon­sti­tu­tion

Sta­bil­i­ty & Storage

Valid for 12 months from date of receipt when stored at -80°C.； Rec­om­mend to aliquot the pro­tein into small­er quan­ti­ties for opti­mal stor­age. Please avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Mouse IL-5 R alpha, His Tag immo­bi­lized on CM5 Chip can bind Biotiny­lat­ed Mouse IL-5, His-Avi Tag with an affin­i­ty con­stant of 0.511 uM as deter­mined in SPR assay (Bia­core T200).

The puri­ty of Biotiny­lat­ed Mouse IL-5 is greater than 95% as deter­mined by SEC-HPLC.

Biotiny­lat­ed Mouse IL-5 on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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